Abstract
Asymmetric functional divergence of paralogues is a key aspect of the traditional model of evolution following duplication. If one gene continues to perform the ancestral function while the other copy evolves a new function then we might expect a period of accelerated sequence evolution following duplication in one of the copies. In keeping with this prediction, many individual examples of asymmetric divergence at the level of protein function have been observed that are accompanied by asymmetric divergence at the sequence level. While several large-scale studies suggest that asymmetric divergence is common across a range of different organisms the degree to which they can be considered to provide an accurate estimate of its prevalence and therefore of the importance of this mode of divergence depends on both the accuracy and power of the methods that have been used. We investigated two methods that can be used to detect asymmetric duplicates using simulated data and real data from Arabidopsis thaliana. One of the methods detects departure from a local molecular clock for amino acid sequences and has been used previously. The second method is novel and tests for different selective constraints along the duplicated lineages using codon models of evolution. This approach is less prone to false positive results but has lower power than the molecular clock method. We find that the power to detect asymmetric divergence is low with both methods unless the effect is strong and report a surprising lack of strong evidence for asymmetric divergence in paralogues derived from the most recent round of genome duplication in Arabidopsis.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Ohno, S.: Evolution by gene duplication. Springer, Heidelberg (1970)
Kondrashov, F., Rogozin, I., Wolfe, K., Koonin, E.: Selection in the evolution of gene duplications. Genome Biology 3 (2002) RESEARCH0008
Hughes, A.: The evolution of functionally novel proteins after gene duplication. Proc. Biol. Sci. 256, 119–124 (1994)
Force, A., Lynch, M., Pickett, F., Amores, A., Yan, Y., Postlethwait, J.: Preservation of duplicate genes by complementary, degenerative mutations. Genetics 151, 1531–1545 (1999)
He, X., Zhang, J.: Rapid subfunctionalization accompanied by prolonged and substantial neofunctionalization in duplicate gene evolution. Genetics 169, 1157–1164 (2005)
Rastogi, S., Liberles, D.: Subfunctionalization of duplicated genes as a transition state to neofunctionalization. BMC Evol Biol. 5, 28 (2005)
Zhang, J., Rosenberg, H., Nei, M.: Positive Darwinian selection after gene duplication in primate ribonuclease genes. Proc. Natl. Acad. Sci. U. S. A. 95, 3708–3713 (1998)
Kellis, M., Birren, B., Lander, E.: Proof and evolutionary analysis of ancient genome duplication in the yeast Saccharomyces cerevisiae. Nature 428, 617–624 (2004)
Zhang, L., Vision, T., Gaut, B.: Patterns of nucleotide substitution among simultaneously duplicated gene pairs in Arabidopsis thaliana. Mol.Biol. Evol. 19, 1464–1473 (2002)
Conant, G., Wagner, A.: Asymmetric sequence divergence of duplicate genes. Genome Res. 13, 2052–2058 (2003)
Blanc, G., Wolfe, K.: Functional divergence of duplicated genes formed by polyploidy during Arabidopsis evolution. Plant Cell 16, 1679–1691 (2004)
Hughes, M., Hughes, A.: Evolution of duplicate genes in a tetraploid animal, Xenopus laevis. Mol. Biol. Evol. 10, 1360–1369 (1993)
Nembaware, V., Crum, K., Kelso, J., Seoighe, C.: Impact of the presence of paralogs on sequence divergence in a set of mouse-human orthologs. Genome Res. 12, 1370–1376 (2002)
Goldman, N., Yang, Z.: A codon-based model of nucleotide substitution for protein-coding DNA sequences. Mol. Biol. Evol. 11, 725–736 (1994)
Yang, Z.: PAML: a program package for phylogenetic analysis by maximum likelihood. Comput. Appl. Biosci. 13, 555–556 (1997)
Wolfe, K., Sharp, P., Li, W.: Mutation rates differ among regions of the mammalian genome. Nature 337, 283–285 (1989)
Nowak, M., Boerlijst, M., Cooke, J., Smith, J.: Evolution of genetic redundancy. Nature 388, 167–171 (1997)
Blanc, G., Hokamp, K., Wolfe, K.: A recent polyploidy superimposed on older large-scale duplications in the Arabidopsis genome. Genome Res. 13, 137–144 (2003)
Vision, T., Brown, D., Tanksley, S.: The origins of genomic duplications in Arabidopsis. Science 290, 2114–2117 (2000)
Bowers, J., Chapman, B., Rong, J., Paterson, A.: Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events. Nature 422, 433–438 (2003)
Blanc, G., Barakat, A., Guyot, R., Cooke, R., Delseny, M.: Extensive duplication and reshuffling in the Arabidopsis genome. Plant Cell 12, 1093–1101 (2000)
Quackenbush, J., Cho, J., Lee, D., Liang, F., Holt, I., Karamycheva, S., Parvizi, B., Pertea, G., Sultana, R., White, J.: The TIGR Gene Indices: analysis of gene transcript sequences in highly sampled eukaryotic species. Nucleic Acids Res. 29, 159–164 (2001)
Altschul, S., Madden, T., Schaffer, A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402 (1997)
Birney, E., Clamp, M., Durbin, R.: GeneWise and Genomewise. Genome Res. 14, 988–995 (2004)
Thompson, J., Higgins, D., Gibson, T.: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673–4680 (1994)
Rice, P., Longden, I., Bleasby, A.: EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 16, 276–277 (2000)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Seoighe, C., Scheffler, K. (2005). Very Low Power to Detect Asymmetric Divergence of Duplicated Genes. In: McLysaght, A., Huson, D.H. (eds) Comparative Genomics. RCG 2005. Lecture Notes in Computer Science(), vol 3678. Springer, Berlin, Heidelberg. https://doi.org/10.1007/11554714_13
Download citation
DOI: https://doi.org/10.1007/11554714_13
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-28932-6
Online ISBN: 978-3-540-31814-9
eBook Packages: Computer ScienceComputer Science (R0)