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Optimization of Insect Cell Based Protein Production Processes - Online Monitoring, Expression Systems, Scale Up

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Yellow Biotechnology II

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 136))

Abstract

Due to the increasing use of insect cell based expression systems in research and industrial recombinant protein production, the development of efficient and reproducible production processes remains a challenging task. In this context, the application of online monitoring techniques is intended to ensure high and reproducible product qualities already during the early phases of process development. In the following chapter, the most common transient and stable insect cell based expression systems are briefly introduced. Novel applications of insect cell based expression systems for the production of insect derived antimicrobial peptides/proteins (AMPs) are discussed using the example of G. mellonella derived gloverin. Suitable in situ sensor techniques for insect cell culture monitoring in disposable and common bioreactor systems are outlined with respect to optical and capacitive sensor concepts. Since scale up of production processes is one of the most critical steps in process development, a conclusive overview is given about scale up aspects for industrial insect cell culture processes.

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Acknowledgments

The researchers would like to thank the Hessen State Ministry of Higher Education, Research and Arts for the financial support within the Hessen initiative for scientific and economic excellence (LOEWE).

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Authors and Affiliations

  1. Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen, Germany

    Damir Druzinec, Denise Salzig & Peter Czermak

  2. Boehringer Ingelheim Vetmedica Inc, St. Joseph, Missouri, USA

    Alexander Brix

  3. Department of Chemical Engineering, University of Technology Berlin, Berlin, Germany

    Matthias Kraume

  4. Fraunhofer Institute of Molecular Biology and Applied Ecology, Giessen, Germany

    Andreas Vilcinskas & Christian Kollewe

  5. Department of Chemical Engineering, Kansas State University, Manhattan, KS, USA

    Peter Czermak

  6. Faculty of Biology and Chemistry, Justus-Liebig-University of Giessen, Giessen, Germany

    Peter Czermak

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  1. Damir Druzinec
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  2. Denise Salzig
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  3. Alexander Brix
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  4. Matthias Kraume
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  5. Andreas Vilcinskas
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  6. Christian Kollewe
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  7. Peter Czermak
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Correspondence to Peter Czermak .

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  1. Bioresources Project Group, Fraunhofer Institute for Molecular Biology, Giessen, Germany

    Andreas Vilcinskas

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Druzinec, D. et al. (2013). Optimization of Insect Cell Based Protein Production Processes - Online Monitoring, Expression Systems, Scale Up. In: Vilcinskas, A. (eds) Yellow Biotechnology II. Advances in Biochemical Engineering/Biotechnology, vol 136. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2013_205

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