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Application of Disposable Bag Bioreactors in Tissue Engineering and for the Production of Therapeutic Agents

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Book cover Bioreactor Systems for Tissue Engineering

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 112))

In order to increase process efficiency, many pharmaceutical and biotechnology companies have introduced disposable bag technology over the last 10 years. Because this technology also greatly reduces the risk of cross-contamination, disposable bags are preferred in applications in which an absolute or improved process safety is a necessity, namely the production of functional tissue for implantation (tissue engineering), the production of human cells for the treatment of cancer and immune system diseases (cellular therapy), the production of viruses for gene therapies, the production of therapeutic proteins, and veterinary as well as human vaccines.

Bioreactors with a pre-sterile cultivation bag made of plastic material are currently used in both development and manufacturing processes primarily operating with animal and human cells at small- and middle-volume scale. Because of their scalability, hydrodynamic expertise and the convincing results of oxygen transport efficiency studies, wave-mixed bioreactors are the most used, together with stirred bag bioreactors and static bags, which have the longest tradition.

Starting with a general overview of disposable bag bioreactors and their main applications, this chapter summarizes the working principles and engineering aspects of bag bioreactors suitable for cell expansion, formation of functional tissue and production of therapeutic agents. Furthermore, results from selected cultivation studies are presented and discussed.

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Authors and Affiliations

  1. Zurich University of Applied Sciences, School of Life Sciences and Facility Management, Institute of Biotechnology, Campus Grüntal, 8820, Switzerland, Wädenswil

    R. Eibl & D. Eibl

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  1. R. Eibl
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Editors and Affiliations

  1. Institute of Technical Chemistry, University of Hannover, Callinstraβe 3, Hannover, 30167, Germany

    Cornelia Kasper

  2. Ludwig Boltzmann Institut für, Klinische und Experimentelle, Traumatologie Donaueschingenstr. 13, Wien, 1200, Austria

    Martijn van Griensven

  3. TU Hamburg-Harburg, Institut für Biotechnologie und Verfahrenstechnik Denickestr. 15, Hamburg, 21073, Germany

    Ralf Pörtner

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Eibl, R., Eibl, D. (2009). Application of Disposable Bag Bioreactors in Tissue Engineering and for the Production of Therapeutic Agents. In: Kasper, C., van Griensven, M., Pörtner, R. (eds) Bioreactor Systems for Tissue Engineering. Advances in Biochemical Engineering/Biotechnology, vol 112. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2008_3

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