Abstract
The ELISPOT assay is widely used for monitoring of immune responses to anti-tumor vaccines. It is a versatile, robust assay, which can be formatted to measure the frequency of antigen-specific T cells in peripheral blood or in culture and to assess the generation of effector cells upon ex vivo priming. The assay performance, results interpretation and its limitations are described.
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Whiteside, T.L. (2005). ELISPOT Assay. In: Nagorsen, D., Marincola, F. (eds) Analyzing T Cell Responses. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3623-X_7
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DOI: https://doi.org/10.1007/1-4020-3623-X_7
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3622-4
Online ISBN: 978-1-4020-3623-1
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