Skip to main content
SpringerLink
Log in
Book cover

Applications of Cell Immobilisation Biotechnology pp 185–196Cite as

Challenges in Cell Encapsulation

  • Chapter

Part of the book series: Focus on Biotechnology ((FOBI,volume 8B))

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bisceglie, V. (1933) Uber die antineoplastische immunitat; heterologe Einpflnzung von Tumoren in Huhner-embryonen. Ztschr. Krebsforsch. 40: 122–140.

    Article  Google Scholar 

  2. Chang, T.M.S. (1964) Semipermeable microcapsules. Science 146: 524–525.

    PubMed  CAS  Google Scholar 

  3. Soon-Shiong, P.; Heintz, R.; Merideth, N.; Yao Quiang, X.; Yao, Z. and Zheng, T. (1994) Insulin independence in a type I diabetic patient after encapsulated islet transplantation. Lancet 343: 950–951.

    Article  PubMed  CAS  Google Scholar 

  4. Hasse, C.; Klock, G.; Schlosser, A.; Zimmermann, U. and Rothmund, M. (1997) Parathyroid allotransplantation without immunosuppression. Lancet 351: 1296–1297.

    Article  Google Scholar 

  5. Orive, G.; Hernández, R.M.; Gascón, A.R.; Calafiore, R.; Chang, T.M.S.; De Vos, P.; Hortelano, G.; Hunkeler, D.; LacÍk, I.; Shapiro, A.M.J. and Pedraz, J.L. (2003) Cell encapsulation: promise and progress. Nat. Med. 9: 104–107.

    Article  PubMed  CAS  Google Scholar 

  6. Orive, G.; Hernández, R.M.; Gascón, A.R.; Igartua, M. and Pedraz, J.L. (2002) Controversies over stem cell research. Trends Biotechnol. 20: 382–387.

    Article  PubMed  CAS  Google Scholar 

  7. Aebischer, P.; Schluep, M.; Déglon, N.; Joseph, J.M.; Hirt, L.; Heyd, B.; Goddard, M.; Hammang, J.P.; Zurn, A.D.; Kato, A.C.; Regli, F. and Baetge, E.E. (1996) Intrathecal delivery of CNTF using encapsulated genetically modified xenogeneic cells in amyotrophic lateral sclerosis patients. Nat. Med. 2: 696–699.

    Article  PubMed  CAS  Google Scholar 

  8. De Vos, P. and Marchetti, P. (2002) Encapsulation of pancreatic islets for transplantation in diabetes: the untouchable islets. Trends Mol. Med. 8: 363–366.

    Article  PubMed  Google Scholar 

  9. Leoni, L. and Desai, T.A. (2001) Nanoporous biocapsules for the encapsulation of insulinoma cells: biotransport and biocompatibility. IEEE Trans. Biomed. Eng. 48: 1335–1341.

    Article  PubMed  CAS  Google Scholar 

  10. Scheneider, B.L.; Schwenter, F.; Pralong, W.F. and Aebischer, P. (2003) Prevention of the initial host immuno-inflammatory response determines the long-term survival of encapsulated myoblasts genetically engineered for erythropoietin delivery. Mol. Ther. 7(4): 506–514.

    Article  CAS  Google Scholar 

  11. Check, E. (2002) Regulators split on gene therapy as patient shows signs of cancer. Nature 419: 545–546.

    Article  PubMed  CAS  Google Scholar 

  12. Emerich, D.F.; Winn, S.R.; Hantraye, P.M.; Peschanski, M.; Chen, E.Y.; Chu, Y.; McDermott, P.; Baetge, E.E. and Kordower, J.H. (1997) Protective effect of encapsulated cells producing neurotrophic factor CNTF in a monkey model of Huntington’s disease. Nature 386:395–399.

    Article  PubMed  CAS  Google Scholar 

  13. Lörh, M.; Hoffmeyer, A.; Kröger, J.C.; Freund, M.; Hain, J.; Holle, A.; Karle, P.; Knöfel, W.T.; Liebe, S.; Müller, P.; Nizze, H.; Renner, M.; Saller, R.M.; Wagner, T.; Hauenstein, K.; Günzburg, W.H. and Salmons B. (2001) Microencapsulated cell-mediated treatment of inoperable pancreatic carcinoma. Lancet 2001 357: 1591–1592.

    Article  Google Scholar 

  14. Angelova, N. and Hunkeler, D. (1999) Rationalizing the design of polymeric biomaterials. Trends Biotechnol. 17: 409–421.

    Article  PubMed  CAS  Google Scholar 

  15. Dauvivier-Kali, V.; Omer, A.; Parent, R.J.; O’Neil, J. and Weir, G.C. (2001) Complete protection of islets against allorejection and autoimmunity by a simple barium-alginate membrane. Diabetes 50: 1698–1705.

    Google Scholar 

  16. Espevik, T.; Otterlei, M.; Skjåk-Bræk, G.; Ryan, L.; Wright, S.D. and Sundan, A. (1993) The involvement of CD14 in stimulation of cytokine production by uronic acid polymers. Eur. J. Immunol. 23: 255–261.

    PubMed  CAS  Google Scholar 

  17. Cole, D.R.; Waterfall, M.; McIntyre, M.; Baird, J.D. (1992) Microencapsulated islet grafts in the BB/E rat: a possible role for cytokines in graft failure. Diabetologia 35: 231–237.

    Article  PubMed  CAS  Google Scholar 

  18. De Vos, P.; De Haan, B.J.; Pater, J. and Van Schilfgaarde, R. (1996) Association between capsule diameter, adequacy of encapsulation, and survival of microencapsulated rat islet allografts. Transplantation 62: 893–899.

    Article  PubMed  Google Scholar 

  19. Soon-Shiong, P.; Otterlei, M.; Skjåk-Bræk, G.; Smidsrød, O.; Heintz, R.; Lanza, P. and Espevik, T. (1991) An immunology basis for the fibrotic reaction to implanted microcapsules. Transplant. Proc. 23: 758–759.

    PubMed  CAS  Google Scholar 

  20. Clayton, H.A.; London, N.J.; Colloby, P.S.; Bell, P.R. and James, R.F. (1991) The effect of capsule composition on the biocompatibility of alginate-poly-l-lysine capsules. J. Microencapsul. 8: 221–233.

    PubMed  CAS  Google Scholar 

  21. Zimmermann, U.; Klöck, G.; Federlin, K.; Haning, K.; Kowaslski, M.; Bretzel, R.G.; Horcher, A.; Entenmann, H.; Siebers, U. and Zekorn, T. (1992) Production of mitogen contamination free alginates with variable rations of mannuronic to guluronic acid by free flow electrophoresis. Electrophoresis 13: 269–274.

    Article  PubMed  CAS  Google Scholar 

  22. Orive, G.; Ponce, S.; Hernández, R.M.; Gascón, A.R.; Igartua, M. and Pedraz, J.L. (2002) Biocompatibility of microcapsules for cell immobilization elaborated with different type of alginates. Biomaterials 23: 3825–3831.

    Article  PubMed  CAS  Google Scholar 

  23. De Vos, P.; De Haan, B.J.; Wolters, G.H.J.; Stubbe, J.H. and Van Schilfgaarde, R. (1997) Improved biocompatibility but limited graft survival after purification of alginate for microencapsulation of pancreatic islets. Diabetologia 40: 262–270.

    Article  PubMed  Google Scholar 

  24. Lim, F. and Sun, A.M. (1980) Microencapsulated islets as bioartificial endocrine pancreas. Science 210: 908–909.

    PubMed  CAS  Google Scholar 

  25. Thu, B.; Bruheim, P.; Espevik, T.; Smidsrød, O.; Soon-Shiong, P. and Skjåk-Bræk, G. (1996) Alginate polycation microcapsules. I. Interaction between alginate and polycation. Biomaterials 17: 1031–1040.

    Article  PubMed  CAS  Google Scholar 

  26. Strand, B.L.; Ryan, L.; Veld, P.I.; Kulseng, B.; Rokstad, A.M.; Skjåk-Bræk, G. and Espevik, T. (2001) Poly-L-lysine induces fibrosis on alginate microcapsules via the induction of cytokines. Cell Transplant. 10: 263–275.

    PubMed  CAS  Google Scholar 

  27. King, A.; Sandler, S. and Andersson, A. (2001) The effect of host factors and capsule composition on the cellular overgrowth on implanted alginate capsules. J. Biomed. Mat. Res. 57: 374–383.

    Article  CAS  Google Scholar 

  28. King, A; Strand, B; Rokstad, AM; Kulseng, B; Andersson A; Skjåk-Bræk, G and Sandler S (2003). Improvement of the biocompatibility of alginate/poly-L-lysine/alginate microcapsules by the use of epimerized alginate as a coating. J. Biomed. Mat. Res. 64:533–539.

    Article  CAS  Google Scholar 

  29. Strand, B.L.; Mørch, Y.A.; Syvertsen, K.R., Espevik, T. and Skjåk-Bræk, G. (2003) Microcapsules made by enzymatically tailored alginate. J. Biomed. Mat. Res. 64: 540–550.

    Article  CAS  Google Scholar 

  30. Kong, H.J.; Smith, M.K. and Mooney, D.J. (2003) Designing alginate hydrogels to maintain viability of immobilised cells. Biomaterials 24: 4023–4029.

    Article  PubMed  CAS  Google Scholar 

  31. Rowley, J.A. and Mooney, D.J. (2002) Alginate type and RGD density control myoblast phenotype. J. Biomed. Mat. Res. 60: 217–223.

    Article  CAS  Google Scholar 

  32. Luca, G.; Calafiore, R.; Basta, G.; Ricci, M.; Calvitti, M.; Neri, L.; Nastruzzi, C.; Becchetti, E.; Capitani, S.; Brunetti, P. and Rossi, C. (2001) Improved function of rat islets upon co-microencapsulation with Sertoly cells in alginate/poly-L-ornithine. AAPS Pharmasci. Tech. 2(3): 1–7.

    Article  Google Scholar 

  33. Orive, G.; Hernández, R.M.; Gascón, A.R.; Igartua, M. and Pedraz, J.L. (2003) Development and optimisation of alginate-PMCG-alginate microcapsules for cell immobilisation. Int. J. Pharm. 259: 57–68.

    Article  PubMed  CAS  Google Scholar 

  34. Cruise, G.M.; Hegre, O.D.; Lamberti, F.V.; Hager, S.R.; Hill, R.; Scharp, D.S. and Hubbell, J.A. (1999) In vitro and in vivo performance of porcine islets encapsulated in interfacially photopolymerized poly(ethylene glycol) diacrylate membranes. Cell Transplant. 8: 293–306.

    PubMed  CAS  Google Scholar 

  35. Schneider, S.; Feilin, P.J.; Slotty, V.; Kampfner, D.; Preuss, S.; Berger, S.; Beyer, J. and Pommersheim, R. (2001) Multilayer capsules: a promising microencapsulation system for transplantation of pancreatic islets. Biomaterials 22: 1961–1970.

    Article  PubMed  CAS  Google Scholar 

  36. Risbud, M.V.; Bhargava, S. and Bhonde, R.R. (2003) In vivo biocompatibility evaluation of cellulose macrocapsules for islet immunoisolation: implications of low molecular weight cut-off. J. Biomed. Mat. Res. 66: 86–92.

    Article  CAS  Google Scholar 

  37. Kobayashi, T.; Aomatsu, Y.; Iwata, H.; Kin, T.; Kanehiro, H.; Hisanaga, M.; Ko, S.; Nagao, M. and Nakajima, Y. (2003) Indefinite islet protection from autoimmune destruction in nonobese diabetic mice by agarose microencapsulation without immunosuppression. Transplantation 75(5): 619–625.

    Article  PubMed  CAS  Google Scholar 

  38. Murphy, F.A. (1996) The public health risk of animal organ and tissue transplantation into humans. Science 273: 746–747.

    PubMed  CAS  Google Scholar 

  39. Orive, G.; Hernández, R.M.; Gascón, A.R.; Igartua, M. and Pedraz, J.L. (2003) Survival of different cell lines in alginate-agarose microcapsules. Eur. J. Pharm. Sci. 18:23–30.

    Article  PubMed  CAS  Google Scholar 

  40. Sommer, B.; Rinsch, C.; Payen, E.; Dalle, B.; Schneider, B.; Déglon, N.; Henri, A.; Beuzard, Y. and Aebischer, P. (2002) Long-term doxycycline-regulated secretion of erythropoietin by encapsulated myoblasts. Mol. Ther. 6(2): 155–161.

    Article  PubMed  CAS  Google Scholar 

  41. Folkman, J. (1989) What is the evidence that tumors are angiogenesis dependent? J. Natl. Cancer Inst. 82: 4–6.

    Google Scholar 

  42. Kim, K.J.; Li, B.; Winer, J.; Armanini, M.; Gillet, N.; Phillips, H.S. and Ferrara, N. (1993) Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumor growth in vivo. Nature 362: 841–844.

    Article  PubMed  CAS  Google Scholar 

  43. Millauer, B.; Schwner, L.K.; Plate, K.H.; Risau, W. and Ullrich, A. (1994) Glioblastoma growth inhibited in vivo by a dominant-negative Flk-1 mutant. Nature 367: 576–579.

    Article  PubMed  CAS  Google Scholar 

  44. Cirone; P.; Bourgeois, M. and Chang, P.L. (2003) Antiangiogenic cancer therapy with microencapsulated cells. Hum. Gene Ther. 14: 1065–1077.

    Article  PubMed  CAS  Google Scholar 

  45. Read, T.A.; Sorensen, D.R.; Mahesparan, R.; Enger, P.Ø.; Timpl, R.; Olsen, B.R.; Hjelstuen, H.B.; Haraldseth, O. and Bjerkvig, R. (2001) Local endostatin treatment of gliomas administered by microencapsulated producer cells. Nat. Biotechnol. 19: 29–34.

    Article  PubMed  CAS  Google Scholar 

  46. Joki, T.; Machluf, M.; Atala, A.; Zhu, J.; Seyfried, N.T.; Dunn, I.F.; Abe, T.; Carroll, R.S.; Black, P.McL. (2001) Continuous release of endostatin from microencapsulated engineered cells for tumor therapy. Nat. Biotechnol. 19: 35–39.

    Article  PubMed  CAS  Google Scholar 

  47. Orive, G.; Hernández, R.M.; Gascón, A.R.; Igartua, M.; Rojas, A. and Pedraz, J.L. (2001) Microencapsulation of an anti VE-cadherin antibody secreting 1B5 hybridoma cells. Biotechnol. Bioeng. 76: 285–294.

    Article  PubMed  CAS  Google Scholar 

  48. Bach, T.L.; Barsigian, C.; Chalupowich, D.G.; Busler, D.; Yaen, C.H.; Grant, D.S. and Martinez, J. (1998) VE-cadherin mediates endothelial cell capillary tube formation in fibrin and collagen gels. Exp. Cell Res. 238: 324–334.

    Article  PubMed  CAS  Google Scholar 

  49. Xu, W.; Liu, L. and Charles, I.G. (2002) Microencapsulated iNOS-expressing cells cause tumor suppression in mice. FASEB J. 16: 213–215.

    PubMed  CAS  Google Scholar 

  50. Lörh, M.; Hummel, F.; Faulmann, G.; Ringel, J.; Saller, R.; Hain, J.; Günzburg, W.H. and Salmons, B. (2002) Microencapsulated, CYP2B1-transfected cells activating ifosfamide at the site of the tumor: the magic bullets of the 21st century. Cancer Chemother. Pharmacol. 49(1): 21–24.

    Google Scholar 

  51. Cirone, P.; Bourgeois, M.; Austin, R.C. and Chang, P.L. (2002) A novel approach to tumor suppression with microencapsulated recombinant cells. Hum. Gene Ther. 13: 1157–1166.

    Article  PubMed  CAS  Google Scholar 

  52. Hillgärtner, M.; Zimmermann, H.; Mimietz, S.; Jork, A.; Thürmer, F.; Scheneider, H.; Nöth, U. and Hasse, C. (1999) Immunoisolation of transplants by entrapment in 19F-labelled alginic gels: production, biocompatibility, stability, and long-term monitoring of functional integrity. Mat. Wiss. U. Werkstofftech. 30: 783–792.

    Article  Google Scholar 

  53. De Vos, P.; Van Hoogmoed, C.G.; De Haan, B.J. and Busscher, H.J. (2002) Tissue responses against immunoisolating alginate-PLL-alginate capsules in the immediate posttransplant period. J. Biomed. Mat. Res. 62:430–437.

    Article  CAS  Google Scholar 

  54. Zimmermann, H.; Hillgärtner, M.; Manz, B.; Feilen, P.; Brunnenmeineier, F.; Leinfelder, U.; Weber, M.; Cramer, H.; Schneider, S.; Hendrich, C.; Volke, F. and Zimmermann, U. (2003) Fabrication of homogeously cross-linked, functional alginate microcapsules validated by NMR-, CLSM-and AFMimaging. Biomaterials 24(12): 2083–2096.

    Article  PubMed  CAS  Google Scholar 

  55. Leinfelder, U.; Brunnenmeier, F.; Cramer, H.; Schiller, J.; Arnold, K.; Vásquez, J.A. and Zimmermann, U. (2003) A highly sensitive cell assay for validation of purification regimes of alginates. Biomaterials 24(23): 4161–4172.

    Article  PubMed  CAS  Google Scholar 

  56. Klemenz, A.; Schwinger, C.; Brandt, J. and Kressler, J. (2003) Investigation of elasto-mechanical properties of alginate microcapsules by scanning acoustic microscopy. J. Biomed. Mat. Res. 65: 237–243.

    Article  CAS  Google Scholar 

  57. Van Raamsdonk, J.M. and Chang, P.L. (2000) Osmotic pressure test: A simple, quantitative method to asses the mechanical stability of alginate microcapsules. J. Biomed. Mater. Res. 54: 264–271.

    Article  Google Scholar 

  58. Lewinska, D.; Rosinski, S.; Hunkeler, D.; Poncelet, D. and Werynski, A. (2002) Mass transfer coefficient in characterization of gel beads and microcapsules. J. Memb. Sci. 5409: 1–8.

    Google Scholar 

  59. Bartkowiak, A. and Hunkeler, D. (1999) New microcapsules based on oligoelectrolyte complexation. Ann. NY Acad. Sci. 875: 36–45.

    Article  PubMed  CAS  Google Scholar 

  60. Saller, R.M.; Indraccolo, S.; Coppola, V.; Esposito, G.; Stange, J.; Mitzner, S.; Heinzmann, U.; Amadori, A.; Salmons, B. and Günzburg, W.H. (2002) Encapsulated cells producing retroviral vectors for in vivo gene therapy. J. Gene Med. 4: 150–160.

    Article  PubMed  Google Scholar 

  61. Peduto, G.; Rinsch, C.; Schneider, B.L.; Rolland, E. and Aebischer, P. (2000) Long-term unresponsiveness to encapsulated xenogeneic myoblasts after transient immunosuppression. Transplantation 70: 78–85.

    PubMed  CAS  Google Scholar 

  62. Ross, C.J.D. and Chang, P.L. (2002) Development of small alginate microcapsules for recombinant gene product delivery to the brain. J. Biomat. Sci. Polym. Edit. 13: 953–962.

    Article  CAS  Google Scholar 

  63. (2002) U.S.Pharmacopeia and National Formulary 1046: 2762–2790.

    Google Scholar 

  64. Orive, G.; Hernández, R.M.; Gascón, A.R.; Igartua, M. and Pedraz, J.L. (2003) Cell microencapsulation technology for biomedical purposes: novel insights and challenges. Trends Pharm. Sci. 24(5): 207–210.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacy & Pharmaceutical Technology, Faculty of Pharmacy, University of the Basque Country, Vitoria, Spain

    Gorka Orive, Rosa Ma Hernández, Alicia R. Gascón & José Luis Pedraz

Authors
  1. Gorka Orive
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rosa Ma Hernández
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alicia R. Gascón
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. José Luis Pedraz
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Belgrade, Belgrade, Serbia and Montenegro

    Viktor Nedović

  2. Free University of Brussels, Brussels, Belgium

    Ronnie Willaert

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Springer

About this chapter

Cite this chapter

Orive, G., Hernández, R.M., Gascón, A.R., Pedraz, J.L. (2005). Challenges in Cell Encapsulation. In: Nedović, V., Willaert, R. (eds) Applications of Cell Immobilisation Biotechnology. Focus on Biotechnology, vol 8B. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3363-X_10

Download citation

Publish with us

Policies and ethics

Search

Navigation