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Finding Disease-Causing Genes

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Abstract

Among the 4000 known human genetic disorders, only a handful of disease-causing genes have been mapped. To locate a gene (say an average of 10,000 bp length) in the midst of a 3.2 billion bp chromosomal DNA is hardly a simple task.

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Chapter 17: Finding Disease-Causing Genes

  • Anand, R. 1992. Yeast artificial chromosomes (YACs) and the analysis of complex genomes. Trends Biotechnol. 10, 35–40.

    Article  PubMed  CAS  Google Scholar 

  • Buckler, A. J., Chang, D. D., Graw, S. L., Brook, D., Haber, D. A., Sharp, P. A., and Housman, D. E. 1991. Exon amplification: a strategy to isolate mammalian genes based on RNA splicing. Proc. Natl. Acad. Sci. USA 88, 4005–4009.

    Article  PubMed  CAS  Google Scholar 

  • Collins, F. S. 1991. Of needles and haystacks: Finding human disease genes by positional cloning. Clin. Res. 39, 615–623.

    PubMed  CAS  Google Scholar 

  • Halaas, J. L., Gajiwala, K. S., Maffei, M., Cohen, S. L., Chaiti, B. T., Rabinowitz, D., Lallone, R. L., Burley, S. K., Friedman, J. M. 1995. Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543–544.

    Article  PubMed  CAS  Google Scholar 

  • Pelleymounter, M. A., Cullen, M. J., Baker, M. B., Hecht, R., Winters, D., Boone, T., and Collins, F. 1995. Effects of the obese gene product on body weight regulation in ob/ob mice. Science 269, 540–543.

    Article  PubMed  CAS  Google Scholar 

  • Poustka, A., Pohl, T. M., Barlow, D. P., Frischauf, A.-M., and Lehrach, H. 1987. Construction and use of human chromosome jumping libraries from NotI-digested DNA. Nature 325, 353–355.

    Article  PubMed  CAS  Google Scholar 

  • White, R., and Lalouel, J.-M. 1988. Chromosome mapping with DNA markers. Sci. Am. 258(2), 40–48.

    Article  PubMed  CAS  Google Scholar 

  • Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., and Friedman, J. M. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature 372, 425–432.

    Article  PubMed  CAS  Google Scholar 

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© 2006 Springer Science+Business Media, Inc.

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(2006). Finding Disease-Causing Genes. In: The ABCs of Gene Cloning. Springer, Boston, MA. https://doi.org/10.1007/0-387-28679-9_17

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