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Opiates Promote T Cell Apoptosis Through JNK and Caspase Pathway

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Book cover Neuroimmune Circuits, Drugs of Abuse, and Infectious Diseases

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 493))

Abstract

Opiate addicts are prone to recurrent infections. In the present study we evaluated the molecular mechanism of opiate-induced T cell apoptosis. Both morphine and DAGO ([D-Ala2,N-Me-Phe4,Gly5-ol]enkephalin) enhanced T cell apoptosis. Morphine as well as DAGO activated c-Jun NH 2 -terminal kinase (JNK) in T cells. Moreover, opiates increased the expression of ATF-2, a specific substrate for JNK and P38 mitogen activated kinases (MAPK). Furthermore, opiates attenuated extracellular signal related kinase (ERK) in T cells. Both morphine and DAGO cleaved pro-caspases 8, 9, and 10 and generated caspases 8, 9 and 10 (active products). Morphine as well as DAGO also cleaved poly- (ADP-ribose) polymerase (PARP) into 116 and 85 kD proteins indicating the activation of caspase-3. These results suggest that opiate-induced T cell apoptosis may be mediated through the JNK cascade and activation of caspases 8 and 3.

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Author information

Authors and Affiliations

  1. Molecular Biology and Experimental Pathology Division of Kidney Diseases and Hypertension, Long Island Jewish Medical Center, New Hyde Park, NY, 11040

    Pravin Singhal, Aditi Kapasi, Krishna Reddy & Nicholas Franki

Authors
  1. Pravin Singhal
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  2. Aditi Kapasi
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  3. Krishna Reddy
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  4. Nicholas Franki
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Editor information

Editors and Affiliations

  1. College of Medicine, University of South Florida, Tampa, Florida

    Herman Friedman  & Thomas W. Klein  & 

  2. Georgia Mental Health Institute, Emory University, Atlanta, Georgia

    John J. Madden

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© 2002 Kluwer Academic Publishers

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Singhal, P., Kapasi, A., Reddy, K., Franki, N. (2002). Opiates Promote T Cell Apoptosis Through JNK and Caspase Pathway. In: Friedman, H., Klein, T.W., Madden, J.J. (eds) Neuroimmune Circuits, Drugs of Abuse, and Infectious Diseases. Advances in Experimental Medicine and Biology, vol 493. Springer, Boston, MA. https://doi.org/10.1007/0-306-47611-8_15

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  • DOI: https://doi.org/10.1007/0-306-47611-8_15

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-46466-9

  • Online ISBN: 978-0-306-47611-2

  • eBook Packages: Springer Book Archive

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