Abstract
Extracellular AMP inhibits cell growth while at the same time stimulates protein and RNA synthesis (Hugo et al., 1992, J. Cell Phys. 153: 539). This principle is applied to establish a two-phase production process for recombinant prethrombin. It is shown that the addition extracellular AMP doubles prethrombin yields and triples cell-specific productivity compared to control cells. Intracellular nucleotide and cyclic AMP measurements together with cell-cycle analyses suggest that growth control by AMP is firstly a result of pyrimidine starvation, and subsequently a result of cyclic-AMP mediated G1 arrest.
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© 1999 Kluwer Academic Publishers
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Bonarius, H.P., Hansen, K., Kongerslev, L. (1999). Extracellular Adenosine 5’ Monophosphate Controls Proliferation and Stimulates Protein Production of rCHO Cells. In: Bernard, A., Griffiths, B., Noé, W., Wurm, F. (eds) Animal Cell Technology: Products from Cells, Cells as Products. Springer, Dordrecht. https://doi.org/10.1007/0-306-46875-1_7
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DOI: https://doi.org/10.1007/0-306-46875-1_7
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-6075-9
Online ISBN: 978-0-306-46875-9
eBook Packages: Springer Book Archive