Abstract
The enhancement of human monoclonal antibody (hMAb) production by retinoic acid (RA) was evaluated using the human-human hybridoma cell line BD9 cultured in serum-free medium. The hMAb production of BD9 hybridomas was enhanced up to eightfold by stimulation with 10 −7 M of RA. Northern blot analysis showed that mRNA levels of hMAb were significantly increased by RA when compared with control without RA. The comparison between intracellular and extracellular hMAb contents by immunoblot analysis showed that hMAb secretion of BD9 hybridomas would be accelerated by RA. These results provide direct evidence for enhancement of hMAb production by RA, suggesting that RA may be effective for mass production of hMAb using BD9 hybridomas.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Aotsuka, Y. and Naito, M. (1991) Enhancing effects of retinoic acid on monoclonal antibody production of human-human hybridomas, Cell. Immunol. 133, 498–505.
Ballow, M., Xiang, S., Wang, W. and Brodsky, L. (1996) The effects of retinoic acid on immunoglobulin synthesis: role of interleukin 6, J. Clin. Immunol. 16, 171–179.
Inoue, Y., Fujisawa, M., Kawamoto, S., Shoji, M., Hashizume, S., Fujii, M., Katakura, Y. and Shirahata, S. (in press) Effectiveness of vitamin A acetate for enhancing the production of lung cancer specific monoclonalantibodies, Cytotechnology.
Kawahara, H., Shirahata, S., Tachibana, H. and Murakami, H. (1992) In vitro immunization of human lymphocytes with human lung cancer cell line A549, Hum. Antibod. Hybridomas 3, 8–13.
Minucci, S., Leid, M., Toyama, R., Saint Jeannet J.P., Peterson, V.J., Horn, V., Ishmael, J.E., Bhattacharyya, N., Dey, A., Dawid, I.B. and Ozato, K. (1997) Retinoid X receptor (RXR) within the RXR-retinoic acid receptor heterodimer binds its ligand and enhances retinoid dependent gene expression, Mol. Cell. Biol. 17, 644–655.
Shoji, M., Kawamoto, S., Sato, S., Kamei, M., Kato, M, Hashizume, S., Seki, K., Yasumoto, K., Nagashima, A., Nakahashi H, Suzuki T, Imai T, Nomoto K and Murakami H (1994) Specific reactivity of human antibody AE6F4 against cancer cells in tissues and sputa from lung cancer patients. Hum. Antibod, Hybridomas 5, 116–122.
VuDac, N., Schoonjans, K., Koskyh, V., Dallongeville, J., Heyman, R.A., Stael, B, and Auwerx, J. (1996) Retinoids increase human apolipoprotein A-II expression through activation of the retinoid X receptor but not the retinoic acid receptor, Mol. Cell. Biol. 16, 3350–3360.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1999 Kluwer Academic Publishers
About this chapter
Cite this chapter
Inoue, Y., Fujisawa, M., Shoji, M., Hashizume, S., Katakuma, Y., Shirahata, S. (1999). Retinoic Acid Enhances Monoclonal Antibody Production of Human-Human Hybridoma BD9. In: Bernard, A., Griffiths, B., Noé, W., Wurm, F. (eds) Animal Cell Technology: Products from Cells, Cells as Products. Springer, Dordrecht. https://doi.org/10.1007/0-306-46875-1_6
Download citation
DOI: https://doi.org/10.1007/0-306-46875-1_6
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-6075-9
Online ISBN: 978-0-306-46875-9
eBook Packages: Springer Book Archive