Abstract
The specific antibody production rate is closely related to the specific ATP production rate. In order to increase the specific antibody production rate by increasing the specific ATP production rate, the effects of oxygen concentration reinforcement (4.7, 6.9 and 10.3 mgl−1 or metabolic intermediate addition (pyruvate, malate and citrate) on ATP production were investigated under glutamine-limited continuous cultivation with a constant specific growth rate. As a result, the specific ATP production rate decreased under high oxygen concentration cultivation. However, the metabolic fluxes related to ATP production were increased and higher specific ATP and antibody production rates were achieved by addition of metabolic intermediates.
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© 2002 Kluwer Academic Publishers
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Omasa, T., Iemura, T., Furuichi, K., Katakura, Y., Kishimoto, M., Suga, Ki. (2002). Energy Metabolism and Antibody Production Based on Flux Analysis in Hybridoma. In: Ikura, K., Nagao, M., Masuda, S., Sasaki, R. (eds) Animal Cell Technology: Challenges for the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/0-306-46869-7_3
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DOI: https://doi.org/10.1007/0-306-46869-7_3
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-5805-3
Online ISBN: 978-0-306-46869-8
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