Conclusions
It should be clear from the above that the calculations described here are at best rough estimations yielding order-of-magnitude values. Even though, the following general conclusions can be drawn. The gradients in stagnant layers surrounding the particles which are characteristic for animal-cell bioreactors are relatively small as compared to the gradients which can be expected in the bulk-liquid phases of the three bioreactors considered, in particular to the gradients in the stagnant layer surrounding the air bubbles. It can be concluded that under almost all circumstances gradients are likely to exist and can be very steep in larger vessels and in particular at high cell densities. The effects of gradients, however, are largely unknown; therefore research on the effects of gradients on specific and volumetric productivities and product quality seems to be an interesting area.
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References
Aunins JG & Henzler H-J (1993) Aeration in cell culture bioreactors. In: Stephanopoulos G (ed) Biotechnology Vol. 3. Bioprocessing, VCH, Weinheim, Germany, 219–280.
Bailey JE & Ollis DF (1986) Biochemical Engineering Fundamentals. McGraw-Hill, Singapore, 460.
Kamen AA, Tom RL, Caron AW, Chavarie C, Massie B & Archambault J. (1991) Culture of insect cells in a helical ribbon impeller bioreactor. Biotechnol. Bioeng. 38: 619–628.
Martens DE, Nollen EAA, Hardeveld M, Van der Velden-de Groot CAM, De Gooijer CD, Beuvery EC & Tramper J (1995) Death rate in a small air-lift loop reactor of Vero cells grown on solid microcarriers and in macroporous microcarriers. Submitted.
Papoutsakis ET (1991) Media additives for protecting animal cells against agitation and aeration damage in bioreactors. Trends in Biotechnol. 12: 45–62.
Sänger P & Deckwer WD (1981) Liquid-solid mass transfer in aerated suspensions. Chem. Eng. J. 22: 179–186.
Sano Y, Yamaguchi N & Adachi T (1974) Mass transfer coefficients for suspended particles in agitated vessels and bubble columns. J. Chem. Eng. Japan 7: 225.
Spier RE & Griffiths B (1984) An examination of the data and concepts germane to the oxygenation of cultured animal cells. Dev. Biol. Stand. 55: 81–92.
Tramper J, De Gooijer CD & Vlak JM (1993) Scale-up considerations and bioreactor development for animal cell cultivation. In: Insect cell culture engineering (Goosen MFA, Daugulis AJ and Faulkner P, eds) Marcel Dekker, New York, USA, 139–177.
van’ t Riet K & Tramper J (1991) Basic Bioreactor Design, Marcel Dekker, New York.
Verlaan P (1987) Modelling and characterization of an air-lift loop bioreactor. PhD thesis, Wageningen Agricultural University, The Netherlands.
Wijffels RH, De Gooijer CD, Schepers AW, Beuling EE, Mallée LR & Tramper J (1995) Dynamic modelling of immobilized Nitrosimonas europaea: implementation of diffusion limitation over expanding microcolonies. Enzyme Microb. Technol. 17: 462–471.
Wijffels RH, de Gooijer CD, Kortekaas S & Tramper J (1991) growth and substrate consumption of Nitrobacter agilis cells immobilized in carrageenan: Part 2. Model evaluation. Biotechnol. Bioeng. 38: 232–240.
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© 1996 Kluwer Academic Publishers
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Tramper, J., Vlak, J., de Gooijer, C. (1996). Oxygen gradients in small and big sparged insect-cell bioreactors. In: Vlak, J., de Gooijer, C., Tramper, J., Miltenburger, H. (eds) Insect Cell Culture: Fundamental and Applied Aspects. Current Applications of Cell Culture Engineering, vol 2. Springer, Dordrecht. https://doi.org/10.1007/0-306-46850-6_20
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