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Exosomes in Urine Biomarker Discovery

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Book cover Urine Proteomics in Kidney Disease Biomarker Discovery

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 845))

Abstract

Nanovesicles present in urine the so-called urinary exosomes have been found to be secreted by every epithelial cell type lining the urinary tract system in human. Urinary exosomes are an appealing source for biomarker discovery as they contain molecular constituents of their cell of origin, including proteins and genetic materials, and they can be isolated in a non-invasive manner. Following the discovery of urinary exosomes in 2004, many studies have been performed using urinary exosomes as a starting material to identify biomarkers in various renal, urogenital, and systemic diseases. Here, we describe the discovery of urinary exosomes and address the issues on the collection, isolation, and normalization of urinary exosomes as well as delineate the systems biology approach to biomarker discovery using urinary exosomes.

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Acknowledgments

This work was supported by the Lundbeck Foundation, Danish Medical Research Foundation, Novo Nordisk Foundation, Carlsberg Foundation, Aarhus University Research Foundation, and the Ratchadapiseksomphot Endowment Fund of Chulalongkorn University (RES560530124-HR).

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Authors and Affiliations

  1. Department of Biomedicine and Center for Interactions of Proteins in Epithelial Transport, Aarhus University, Aarhus, Denmark

    Alyssa R. Huebner, Robert A. Fenton & Trairak Pisitkun

  2. Systems Biology Center, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

    Poorichaya Somparn & Trairak Pisitkun

  3. Center of Excellence in Immunology and Immune Mediated Diseases, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

    Thitima Benjachat, Asada Leelahavanichkul & Yingyos Avihingsanon

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  1. Alyssa R. Huebner
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  3. Thitima Benjachat
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Correspondence to Trairak Pisitkun .

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  1. Pathophysiology, Peking Union Medical College & Inst. Basic Medical Sciences Chinese Academy of Medical Sciences, Beijing, China

    Youhe Gao

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Huebner, A.R. et al. (2015). Exosomes in Urine Biomarker Discovery. In: Gao, Y. (eds) Urine Proteomics in Kidney Disease Biomarker Discovery. Advances in Experimental Medicine and Biology, vol 845. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9523-4_5

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