Are Chlorophyll-Carotenoid Interactions Responsible for Rapidly Reversible Non-Photochemical Fluorescence Quenching?
- Herbert van AmerongenAffiliated withLaboratory of Biophysics, Wageningen University Email author
Photoprotective thermal energy dissipation (as assessed via non-photochemical quenching of singlet-excited chlorophyll a, NPQ) in plants is driven by various mechanisms occurring over different time scales. The rapid and reversible part of NPQ, also called qE (for energy-dependent quenching), was demonstrated to correlate with the twisting of a neoxanthin molecule in the light-harvesting antenna as observed by resonance Raman spectroscopy (Nature 450: 575–578, 2007). Interestingly, the extent of fluorescence quenching correlates with the change in Raman signal in different situations: during NPQ in vivo, during fluorescence quenching upon aggregation of LHCII (the major light-harvesting complex in plants), and in crystals of LHCII. In the same study, it was proposed that the quenching is caused by excitation energy transfer from chlorophyll a to lutein in LHCII after a structural change that correlates with the twisting of the neoxanthin. However, this view has been challenged by others for different reasons. Here we discuss the arguments in favor and against this mechanism. A short overview is given of the spectroscopic data on chlorophyll-carotenoid interactions in plant light-harvesting systems, the changes in interactions upon aggregation or crystallization, and the possible relationship to the mechanism of NPQ.
- Are Chlorophyll-Carotenoid Interactions Responsible for Rapidly Reversible Non-Photochemical Fluorescence Quenching?
- Book Title
- Non-Photochemical Quenching and Energy Dissipation in Plants, Algae and Cyanobacteria
- pp 333-342
- Print ISBN
- Online ISBN
- Series Title
- Advances in Photosynthesis and Respiration
- Series Volume
- Series Subtitle
- Including Bioenergy and Related Processes
- Series ISSN
- Springer Netherlands
- Copyright Holder
- Springer Science+Business Media Dordrecht
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- Editor Affiliations
- 3. Ecology and Evolutionary Biology, University of Colorado, Boulder
- 4. Laboratory of Photosynthetic Membranes Biological Research Center, Institute of Plant Biology Hungarian Academy of Sciences
- 5. Ecology and Evolutionary Biology, University of Colorado
- 6. Department of Plant Biology 265 Morrill Hall, MC-116, University of Illinois at Urbana-Champaign
- Author Affiliations
- 7. Laboratory of Biophysics, Wageningen University, 8128, Wageningen, 6700 ET, The Netherlands
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