Chapter

Intracellular Delivery II

Volume 7 of the series Fundamental Biomedical Technologies pp 363-401

Date:

Nanodiamonds as Intracellular Probes for Imaging in Biology and Medicine

  • Jitka SlegerovaAffiliated withLaboratory of Synthetic Nanochemistry, Institute of Organic Chemistry and Biochemistry AS CR v. v. i.
  • , Ivan RehorAffiliated withLaboratory of Synthetic Nanochemistry, Institute of Organic Chemistry and Biochemistry AS CR v. v. i.
  • , Jan HavlikAffiliated withLaboratory of Synthetic Nanochemistry, Institute of Organic Chemistry and Biochemistry AS CR v. v. i.
  • , Helena RaabovaAffiliated withLaboratory of Synthetic Nanochemistry, Institute of Organic Chemistry and Biochemistry AS CR v. v. i.
  • , Eva MuchovaAffiliated withLaboratory of Synthetic Nanochemistry, Institute of Organic Chemistry and Biochemistry AS CR v. v. i.
  • , Petr CiglerAffiliated withLaboratory of Synthetic Nanochemistry, Institute of Organic Chemistry and Biochemistry AS CR v. v. i. Email author 

* Final gross prices may vary according to local VAT.

Get Access

Abstract

In recent years, diamond nanoparticles have received a great deal of attention due to their unique photophysical and biological properties. Nanodiamonds (NDs) show low toxicity and are considered to be a highly biocompatible carbon nanomaterial useful in a wide range of applications. Thanks to their ability to accommodate nitrogen-vacancy (N-V) color centers, NDs are a prime example of non-photobleachable fluorescent labels and nanosensors. Here, we present a survey of ND applications in biology and medicine with an emphasis on bio-imaging. We focus on distinguishing the properties of detonation NDs and high-pressure high-temperature (HPHT) NDs and describing their physicochemical properties, structure and possible modifications by small molecules and biomolecules. We summarize and critically evaluate in vitro and in vivo data on ND toxicity and biocompatibility, cellular internalization, localization and targeting by surface-attached ligands. We discuss current achievements in bioimaging using fluorescent NDs and the potential of NDs in diagnostics and drug delivery.

Keywords

Nanodiamond Fluorescence Nitrogen-vacancy center Intracellular probe Bioimaging Biocompatibility Targeting Cellular internalization Drug delivery