Isolation and Mode of Action of Ptr Chlorosis Toxin From Pyrenophora Tritici-Repentis

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Pyrenophora tritici-repentis (Ptr) induces differentially tan necrosis and extensive chlorosis in its hexaploid wheat host. The necrosis symptom was shown to be associated with the Ptr necrosis toxin, a 14 kd protein [3], This toxin has been previously characterized by several independent laboratories and its encoding gene recently cloned [1,2]. A chlorosis inducing host-specific toxin, termed the Ptr chlorosis toxin, was isolated by our group from race 5 of Ptr. This toxin was found to be a hydrophilic protein, stable to exposure to organics [4] The objectives of the present investigations were to (1) develop an effective method to purify Ptr chlorosis toxin, and to (2) elucidate its mode of action.