1995, pp 433-447

Immunoassay Methods of Plant Hormone Analysis

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The use of immunoassays in the analysis of plant hormones has increased significantly since the founding work of Weiler and associates (58). Immunoassays are based on the ability of animals to produce proteins (antibodies) which recognize and bind to specific compounds (antigens) foreign to the animal. The specificity and sensitivity of antibodies make immunoassays attractive for the quantitation of plant hormones, which usually occur at low concentrations. Small amounts of plant tissue can thus be used in the assay, and, in addition, large numbers of samples can be processed in a brief period of time. Although some purification of extracts is generally required for the immunoassays, it is generally less than the more extensive clean-up needed in physico-chemical methods.