Correlation between interphase and metaphase chromosome arrangements as studied by laser-uv-microbeam experiments
In spite of many attempts at resolution, the problem of the chromosome topography in interphase nuclei and its possible functional significance (Lewin, 1981; Vogel and Krüger, 1983) has remained a matter of great controversy and uncertainty (for reviews see Comings,1980; Avivi & Feldman, 1980). In particular, the relationship between metaphase and interphase arrangements of chromosomes is still largely unknown. A solution of this problem seems especially urgent, since the spatial relationship of chromosomes at metaphase has been extensively studied in many species during the last twenty years. Recently, Bennett and coworkers have demonstrated a highly ordered arrangement of centromeres in metaphase plates of certain plant species (Bennett, 1983) and stimulated fresh interest in the development of techniques for mapping the arrangements of individual chromosomes directly within the interphase nucleus. In this paper we describe a laser-uv-microbeam approach which allows selective microirradiation of a small amount of chromatin in the interphase nucleus of cultured cells and study of its distribution in chromosomes during the subsequent mitosis. Alternatively, chromatin can be microirradiated during mitosis and its distribution can be studied in the subsequent interphase nucleus.
- Correlation between interphase and metaphase chromosome arrangements as studied by laser-uv-microbeam experiments
- Book Title
- Chromosomes Today
- Book Subtitle
- Volume 8 Proceedings of the Eighth International Chromosome Conference held in Lübeck, West Germnay, 21–24 September 1983
- pp 203-212
- Print ISBN
- Online ISBN
- Springer Netherlands
- Copyright Holder
- Springer Science+Business Media B.V.
- Additional Links
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- Editor Affiliations
- 1. Plant Breeding Institute
- 2. Institut für Pathologie, Medizinische Hochschule Lübeck
- 3. Institut für Humangenetik und Anthropologie der Universität
- Author Affiliations
- 4. Institute of Anthropology and Human Genetics, University of Heidelberg, D-6900, Heidelberg, Germany
- 5. Department of Pathology, Kyoto Prefectural, University of Medicine, Kyoto, Japan
- 6. Institute of Applied Physics I, University of Heidelberg, D-6900, Heidelberg, Germany
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