Chapter

Metallomics and the Cell

Volume 12 of the series Metal Ions in Life Sciences pp 241-278

Date:

The Iron Metallome in Eukaryotic Organisms

  • Adrienne C. DlouhyAffiliated withDepartment of Chemistry and Biochemistry, University of South Carolina
  • , Caryn E. OuttenAffiliated withDepartment of Chemistry and Biochemistry, University of South Carolina Email author 

Abstract

This chapter is focused on the iron metallome in eukaryotes at the cellular and subcellular level, including properties, utilization in metalloproteins, trafficking, storage, and regulation of these processes. Studies in the model eukaryote Saccharomyces cerevisiae and mammalian cells will be highlighted. The discussion of iron properties will center on the speciation and localization of intracellular iron as well as the cellular and molecular mechanisms for coping with both low iron bioavailability and iron toxicity. The section on iron metalloproteins will emphasize heme, iron-sulfur cluster, and non-heme iron centers, particularly their cellular roles and mechanisms of assembly. The section on iron uptake, trafficking, and storage will compare methods used by yeast and mammalian cells to import iron, how this iron is brought into various organelles, and types of iron storage proteins. Regulation of these processes will be compared between yeast and mammalian cells at the transcriptional, post-transcriptional, and post-translational levels.

Keywords

eukaryote heme iron metallome iron-sulfur cluster iron trafficking metal homeostasis