Determination of Cadmium in Biological Samples
- Katrin KlotzAffiliated withInstitute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Friedrich-Alexander University (FAU) of Erlangen-Nuremberg Email author
- , Wobbeke WeistenhöferAffiliated withInstitute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Friedrich-Alexander University (FAU) of Erlangen-Nuremberg
- , Hans DrexlerAffiliated withInstitute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Friedrich-Alexander University (FAU) of Erlangen-Nuremberg
Analyses of cadmium concentrations in biological material are performed using inductively coupled plasma mass spectrometry (ICP-MS) and atomic absorption spectrometry (AAS), but also electrochemical methods, neutron activation analysis (NAA), and X-ray fluorescence spectrometry (XRF). The predominant sample matrices include blood, plasma, serum, and urine, as well as hair, saliva, and tissue of kidney cortex, lung, and liver. While cadmium in blood reveals rather the recent exposure situation, cadmium in urine reflects the body burden and is an indicator for the cumulative long term exposure.
After chronic exposure, cadmium accumulates in the human body and causes kidney diseases, especially lesions of proximal tubular cells. A tubular proteinuria causes an increase in urinary excretion of microproteins. Excretions of retinol binding protein (RBP), β2-microglobulin (β2-M), and α1-microglobulin are validated biomarkers for analyzing cadmium effects. For this purpose, immunological procedures such as ELISA, and radio- and latex-immunoassays are used.
However, proteinuria is not specific to cadmium, but can also occur after exposure to other nephrotoxic agents or due to various kidney diseases. In summary, cadmium in urine and blood are the most specific biomarkers of cadmium exposure. A combination of parameters of exposure (cadmium in blood, cadmium in urine) and parameters of effect (e.g., β2-M, RBP) is required to reveal cadmium-induced nephrological effects.
KeywordsAAS analysis biomonitoring blood cadmium ICP-MS immunoassay urine
- Determination of Cadmium in Biological Samples
- Book Title
- Cadmium: From Toxicity to Essentiality
- pp 85-98
- Print ISBN
- Online ISBN
- Series Title
- Metal Ions in Life Sciences
- Series Volume
- Series ISSN
- Springer Netherlands
- Copyright Holder
- Springer Science+Business Media Dordrecht
- Additional Links
- Industry Sectors
- Editor Affiliations
- 2. Inorganic Chemistry, Chemistry, Universität Basel
- 3. Inst. Anorganische Chemie, Universität Basel
- 4. Institute of Inorganic Chemistry, University of Zürich
- Author Affiliations
- 1. Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Friedrich-Alexander University (FAU) of Erlangen-Nuremberg, Schillerstr. 25/29, D-91052, Erlangen, Germany
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