Chapter

Reprogramming Microbial Metabolic Pathways

Volume 64 of the series Subcellular Biochemistry pp 181-201

Date:

Tunable Promoters in Synthetic and Systems Biology

  • Tore DehliAffiliated withCenter for Systems Microbiology, Department of Systems Biology, Technical University of Denmark
  • , Christian SolemAffiliated withCenter for Systems Microbiology, Department of Systems Biology, Technical University of Denmark
  • , Peter Ruhdal JensenAffiliated withCenter for Systems Microbiology, Department of Systems Biology, Technical University of Denmark Email author 

Abstract

Synthetic and systems biologists need standardized, modular and orthogonal tools yielding predictable functions in vivo. In systems biology such tools are needed to quantitatively analyze the behavior of biological systems while the efficient engineering of artificial gene networks is central in synthetic biology. A number of tools exist to manipulate the steps in between gene sequence and functional protein in living cells, but out of these the most straight-forward approach is to alter the gene expression level by manipulating the promoter sequence. Some of the promoter tuning tools available for accomplishing such altered gene expression levels are discussed here along with examples of their use, and ideas for new tools are described. The road ahead looks very promising for synthetic and systems biologists as tools to achieve just about anything in terms of tuning and timing multiple gene expression levels using libraries of synthetic promoters now exist.

Keywords

Metabolic engineering Standardization, orthogonality and modularity Synthetic and systems biology Synthetic gene networks Synthetic promoter libraries