Chapter

Aging Research in Yeast

Volume 57 of the series Subcellular Biochemistry pp 251-289

Date:

Genome-Wide Analysis of Yeast Aging

  • George L. SutphinAffiliated withDepartment of Pathology and the Molecular and Cellular Biology Program, University of Washington
  • , Brady A. OlsenAffiliated withDepartment of Pathology, University of Washington
  • , Brian K. KennedyAffiliated withBuck Institute
  • , Matt KaeberleinAffiliated withDepartment of Pathology, University of Washington Email author 

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Abstract

In the past several decades the budding yeast Saccharomyces cerevisiae has emerged as a prominent model for aging research. The creation of a single-gene deletion collection covering the majority of open reading frames in the yeast genome and advances in genomic technologies have opened yeast research to genome-scale screens for a variety of phenotypes. A number of screens have been performed looking for genes that modify secondary age-associated phenotypes such as stress resistance or growth rate. More recently, moderate-throughput methods for measuring replicative life span and high-throughput methods for measuring chronological life span have allowed for the first unbiased screens aimed at directly identifying genes involved in determining yeast longevity. In this chapter we discuss large-scale life span studies performed in yeast and their implications for research related to the basic biology of aging.

Keywords

Acetic acid Apoptosis Asymmetric segregation Chronological life span Counter flow centrifugation elutriation (CCE) Dietary restriction (DR) Genome-wide Genomics High-throughput Loss of heterozygosity (LOH) Metabolomics Microarrays Mitochondria Mitochondrial back-signaling Mother Enrichment Program (MEP) Oxidative damage Proteomics Replicative life span Retrograde response Ribosomal DNA (rDNA) Sirtuins Target of rapamycin (TOR) signaling Translation Worms Yeast Outgrowth Data Analysis (YODA)