Ultrasound Activated Nano-Encapsulated Targeted Drug Delivery and Tumour Cell Poration
- Dana GourevichAffiliated withInstitute of Medical Science and Technology, University of DundeeCapsuTech Ltd
- , Bjoern GeroldAffiliated withInstitute of Medical Science and Technology, University of DundeeInSightec Ltd Email author
- , Fabian ArdittiAffiliated withCapsuTech Ltd
- , Doudou XuAffiliated withInstitute of Medical Science and Technology, University of DundeeCapsuTech Ltd
- , Dun LiuAffiliated withInstitute of Medical Science and Technology, University of DundeeCapsuTech Ltd
- , Alex VolovickAffiliated withInstitute of Medical Science and Technology, University of DundeeInSightec Ltd
- , Lijun WangAffiliated withInstitute of Medical Science and Technology, University of Dundee
- , Yoav MedanAffiliated withInSightec Ltd
- , Jallal GnaimAffiliated withCapsuTech Ltd
- and 3 more
- , Paul PrenticeAffiliated withInstitute of Medical Science and Technology, University of Dundee
- , Sandy CochranAffiliated withInstitute of Medical Science and Technology, University of Dundee
- , Andreas MelzerAffiliated withCapsuTech Ltd
- Show less
Introduction: Recently, ultrasonic drug release has been a focus of many research groups for stimuli responsive drug release. It has been demonstrated that a focused ultrasound (FUS) beam rapidly increases the temperature at the focused tissue area. One potential mechanism of drug targeting is to utilize the induced heat to release or increase penetration of chemotherapy to cancer cells. The efficiency of targeted drug delivery may increase by using FUS beam in conjugation with nano-encapsulated drug carriers.
The aim of this study is to investigate the effect of heat and ultrasound on the cellular uptake and therapeutic efficacy of an anticancer drug using Magnetic Resonance Imaging guided Focused Ultrasound (MRgFUS).
Materials and Methods: Human KB cells (CCL-17 cells) were seeded into 96-well plates and heat treated at 37–55°C for 2–10 min. Cell viability was determined using the colorimetric MTT assay. The cells were also subjected to MRgFUS and the degree of cell viability was determined. These experiments were conducted using an ExAblate 2000 system (InSightec, Haifa, Israel) and a GE 1.5 T MRI system, software release 15.
Results: We have observed a significant decrease in human KB cell viability due to heat (>41°C) in the presence of Doxorubicin (DOX), in comparison with DOX at normal culture temperature (37°C). The synergistic effect of heat with DOX may be explained by several mechanisms. One potential mechanism may be increased penetration of DOX to the cells during heating. In addition, we have shown that ultrasound induced cavitation causes cell necrosis.
Discussion and Future work: Further investigation is required to optimize the potential of MRgFUS to enhance cellular uptake of therapeutic agents. A novel delivery nano-vehicle developed by CapsuTech will be investigated with MRgFUS for its potential as a stimuli responsive delivery system.
Acknowledgments: This work is supported by an EU FP7 Industrial Academia Partnership Pathway IAPP.
KeywordsCancer Cell culture HIFU Targeted drug delivery Ultrasound
- Ultrasound Activated Nano-Encapsulated Targeted Drug Delivery and Tumour Cell Poration
- Book Title
- Nano-Biotechnology for Biomedical and Diagnostic Research
- pp 135-144
- Print ISBN
- Online ISBN
- Series Title
- Advances in Experimental Medicine and Biology
- Series Volume
- Series ISSN
- Springer Netherlands
- Copyright Holder
- Springer Science+Business Media B.V.
- Additional Links
- Cell culture
- Targeted drug delivery
- Industry Sectors
- eBook Packages
- Editor Affiliations
- ID1. Israel Institute for Biological Research
- ID2. Israel Institute for Biological Research
- ID3. Israel Institute for Biological Research
- ID4. Israel Institute for Biological Research
- Author Affiliations
- 1. Institute of Medical Science and Technology, University of Dundee, Dundee, UK
- 2. CapsuTech Ltd, Nazareth, Israel
- 3. InSightec Ltd, Haifa, Israel
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