Chapter

Cell and Organ Printing

pp 95-113

Date:

High-Throughput Biological Laser Printing: Droplet Ejection Mechanism, Integration of a Dedicated Workstation, and Bioprinting of Cells and Biomaterials

  • Fabien GuillemotAffiliated withINSERM, U577University of Victor Segalen Bordeaux 2 Email author 
  • , Bertrand GuillotinAffiliated withINSERM, U577University of Victor Segalen Bordeaux 2
  • , Sylvain CatrosAffiliated withINSERM, U577University of Victor Segalen Bordeaux 2
  • , Agnès SouquetAffiliated withINSERM, U577University of Victor Segalen Bordeaux 2
  • , Candice MezelAffiliated withCentre Lasers Intenses et Applications, UMR 5107 CEA V CNRS – Université Bordeaux 1
  • , Virginie KeriquelAffiliated withINSERM, U577University of Victor Segalen Bordeaux 2
  • , Ludovic HalloAffiliated withCentre Lasers Intenses et Applications, UMR 5107 CEA V CNRS – Université Bordeaux 1
  • , Jean-Christophe FricainAffiliated withINSERM, U577University of Victor Segalen Bordeaux 2
  • , Joëlle AmedeeAffiliated withINSERM, U577University of Victor Segalen Bordeaux 2

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Abstract

High-Throughput Biological Laser Printing (HT BioLP) requires taking into account spatio-temporal proximity of laser pulses (that means pulse-to-pulse distance and laser pulse frequency). The droplet ejection mechanism is indeed governed by vapor bubble dynamics (bubble growth and collapsing) and it is thus related to both the condition of laser irradiation and the rheological properties of the liquid film (viscosity, surface tension). We present a rapid prototyping workstation which has been designed for HT BioLP applications. It is equipped with an infra-red pulsed laser (pulse duration = 30 ns, wavelength = 1,064 nm, f = 1–100 kHz), galvanometric mirrors (scanning speed up to 2,000 mm/s), micrometric translation stages (x, y, z) and a dedicated software. Then, after describing experimental conditions leading to the high resolution printing (including cell density, laser parameters, etc.) of biological components, we present some typical multi-component and 3D printings achieved using this workstation. Finally, considering different criteria (speed, inoquity, etc.) the potentiality of HT BioLP is discussed as an alternative technology in Tissue Engineering applications.