Chapter

Arterial Chemoreceptors

Volume 648 of the series Advances in Experimental Medicine and Biology pp 403-410

Nitric Oxide in the Solitary Tract Nucleus (STn) Modulates Glucose Homeostasis and FOS-ir Expression After Carotid Chemoreceptor Stimulation

  • M. LemusAffiliated withCentro Universitario de Investigaciones Biomédicas, Universidad de Colima
  • , S. MonteroAffiliated withCentro Universitario de Investigaciones Biomédicas, Universidad de Colima
  • , S. LuquínAffiliated withCentro de Investigaciones de Occidente
  • , J. GarcíaAffiliated withFacultad de Medicina, Universidad de Guadalajara
  • , E. Roces De Álvarez-BuyllaAffiliated withUniversidad de Colima Av. 25 de Julio S/N, Col. villas de San Sebastián Email author 

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Abstract

We evaluate in rats the role of NO in the solitary tract nucleus (STn) after an anoxic stimulus to carotid body chemoreceptor cells (CChrc) with cyanide (NaCN), on the hyperglycemic reflex with glucose retention by the brain (BGR) and FOS expression (FOS-ir) in the STn. The results suggest that nitroxidergic pathways in the STn may play an important role in glucose homeostasis. A NO donor such as sodium nitroprusside (NPS) in the STn before CChrc stimulation increased arterial glucose level and significantly decreased BGR. NPS also induced a higher FOS-ir expression in STn neurons when compared to neurons in control rats that only received artificial cerebrospinal fluid (aCSF) before CChrc stimulation. In contrast, a selective NOS inhibitor such as Nω-nitro-L-arginine methyl ester (L-NAME) in the STn before CChrc stimulation resulted in an increase of both, systemic glucose and BGR above control values. In this case, the number of FOS-ir positive neurons in the STn decreased when compared to control or to NPS experiments. FOS-ir expression in brainstem cells suggests that CChrc stimulation activates nitroxidergic pathways in the STn to regulate peripheral and central glucose homeostasis. The study of these functionally defined cells will be important to understand brain glucose homeostasis.

Keywords

Nitric oxide solitary tract nucleus Glucose Sodium nitroprusside Fos Cerebrospinal fluid Carotid chemoreceptor