Chapter

African Horse Sickness

pp 329-336

Western immunoblotting as a method for the detection of African horse sickness virus protein-specific antibodies: Differentiation between infected and vaccinated horses

  • S. Idrissi BougrineAffiliated withDépartment de Microbiologie Immunologie Maladies Contagieuses, Institut Agronomique et Vétérinaire Hassan II
  • , O. Fassi FihriAffiliated withDépartement de Microbiologie Immunologie Maladies Contagieuses, Institut Agronomique et Vétérinaire Hassan II
  • , M. M. Fassi FehriAffiliated withDépartement de Microbiologie Immunologie Maladies Contagieuses, Institut Agronomique et Vétérinaire Hassan II

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Summary

A Western immunoblotting procedure has been developed for the detection of African horse sickness virus (AHSV) protein-specific antibody responses. This assay readily identifies antibodies specific for at least 4 distinct, AHSV proteins, including VP5, NS1, NS2 and NS3/NS3a. By using the AHSV non-structural proteins as ‘markers’, the Western blotting procedure could be employed to provide a reliable means of discriminating between animals vaccinated with a purified, inactivated AHSV vaccine and those either naturally infected or vaccinated with a live, attenuated AHSV vaccine.