Chapter

Spheroids in Cancer Research

Volume 95 of the series Recent Results in Cancer Research pp 1-23

Liquid-Overlay Culture of Cellular Spheroids

  • J. CarlssonAffiliated withDepartment of Radiation Biology (FOA 4), National Defence Research Institute
  • , J. M. YuhasAffiliated withDepartment of Radiation Biology (FOA 4), National Defence Research Institute

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Abstract

The aim of this work has been to develop realistic and easily handled models of tumor growth, from which increased knowledge of the physical and chemical factors that govern or sometimes disturb cell proliferation may be obtained. Emphasis has been laid mainly on the properties of cultured spheroids originating from mammalian cells. The three-dimensional arrangement affects the concentration of oxygen and other substances and invokes, for cells inside the spheriods, physical, chemical, and nutritional stress factors that are probably typical of poorly vascularized areas of solid tumors. Increased knowledge of this situation is important, because it is believed to determine radiation sensivity and also the effects of cytotoxic substances.