Viral Expression Vectors

Volume 158 of the series Current Topics in Microbiology and Immunology pp 67-95

Epstein-Barr Virus Based Expression Vectors

  • R. F. MargolskeeAffiliated withDepartment of Neurosciences, Roche Institute of Molecular Biology, Roche Research Center

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Eukaryotic viral vectors have played an important role in the expression and analysis of many eukaryotic genes. The field is growing at a rapid pace and is by no means mature (for contrast see the reports from the first and second Banbury Conferences on Eukaryotic Viral Vectors, Gluzman 1982; Gluzman and Hughes 1988). Although in theory it should be possible to develop a eukaryotic vector from any virus, practical considerations have led to concentration on a few well-characterized viruses, e.g., papovaviruses, adenoviruses, herpesviruses, and retroviruses. As the understanding of the underlying biology of the virus’ life cycle has matured, so too has the sophistication of specific virus based vectors. Frequently the development of virus vectors has been instrumental in dissecting the functional elements of the parent virus. Present-day eukaryotic vectors are a collection of eukaryotic elements from many different cellular and viral sources. Unfortunately, there are no simple rules for constructing the best eukaryotic vector, hence much of this field has developed from empirical trials. Furthermore, no single vector presently exists that provides a solution for all situations that require a eukaryotic vector.