Cytokines in Hemopoiesis, Oncology, and AIDS

pp 305-309

Mast Cell Growth-Enhancing Activity (MEA) is a Potent Inducer of Interleukin-6 Production in a Murine Interleukin-3-Dependent Mast Cell Line

  • L. Hültner
  • , J. Moeller
  • , P. Dörmer

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Mouse bone marrow-derived mast cells (BMMC) are known to be stimulated to grow in vitro in response to interleukin-3 (IL-3) [1] and IL-4 [2]. In our laboratory we have established IL-3-/IL-4-responsive BMMC lines using pokeweed mitogen (PWM)-stimulated spleen cell-conditioned medium (SCM) as a source of mast cell growth factors [3]. Recently, we detected in SCM a novel mast cell growth-enhancing activity (MEA) for permanent factor-dependent BMMC lines [3]. The biological activity of MEA was originally assessed by its capacity to synergistically enhance the proliferation of established factor-dependent BMMC lines stimulated with saturating amounts of IL-3 in the presence of a neutralizing monoclonal anti-IL-4 antibody (11B11) [3]. MEA was characterized as a glycoprotein (37–44 kD; pI 6.2–7.3) different from PWM, colony-stimulating factors (GM-CSF, G- CSF, CSF-1), interleukins (IL-l-IL-7), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), transforming growth factor (TGF)-α, nerve growth factor (NGF), and erythropoietin [3, 4]. It was partially purified and completely separated from IL-4 and IL-3 using cation exchange and Procion Red affinity chromatography [4].