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Point Source Digital In-Line Holographic Microscopy

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Book cover Coherent Light Microscopy

Part of the book series: Springer Series in Surface Sciences ((SSSUR,volume 46))

Abstract

Point source digital in-line holography with numerical reconstruction has been developed into a new microscopy, specifically for microfluidic and biological applications, that routinely achieves both lateral and depth resolution at the submicron level in 3-D imaging. This review will cover the history of this field and give details of the theoretical and experimental background. Numerous examples from microfluidics and biology will demonstrate the capabilities of this new microscopy. The motion of many objects such as living cells in water can be tracked in 3-D at subsecond rates. Microfluidic applications include sedimentation of suspensions, fluid motion around micron-sized objects in channels, motion of spheres, and formation of bubbles. Immersion DIHM will be reviewed which effectively does holography in the UV. Lastly, a submersible version of the microscope will be introduced that allows the in situ study of marine life in real time in the ocean and shows images and films obtained in sea trials.

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Author information

Authors and Affiliations

  1. Department of Physics and Atmospheric Science, Dalhousie University, Halifax, NS, Canada

    Manfred H. Jericho

  2. Department of Physics and Atmospheric Science, Dalhousie University, Halifax, NS, B3H 3J5, Canada

    H. Jürgen Kreuzer

Authors
  1. Manfred H. Jericho
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  2. H. Jürgen Kreuzer
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Correspondence to H. Jürgen Kreuzer .

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Editors and Affiliations

  1. Ist. Nazionale di Ottica Applicata, CNR, Via Campi Flegrei 34, Pozzuoli, 80078, Napoli, Italy

    Pietro Ferraro

  2. Dept. Biomedical Engineering, Duke University, Durham, 27708-0292, North Carolina, USA

    Adam Wax

  3. Dept. Engineering, Bar-Ilan University, Ramat Gan, 52900, Israel

    Zeev Zalevsky

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Jericho, M.H., Jürgen Kreuzer, H. (2011). Point Source Digital In-Line Holographic Microscopy . In: Ferraro, P., Wax, A., Zalevsky, Z. (eds) Coherent Light Microscopy. Springer Series in Surface Sciences, vol 46. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-15813-1_1

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