Abstract
Tissue engineering offers new strategies in developing treatments for the repair of hyaline cartilage. Autologous chondrocyte implantation (ACI) method is initially based on cultivation of chondrocytes in vitro, followed by injection of the obtained cell suspension below the periosteal flap into the cartilage defect. The problem that appears by cell expansion in a monolayer culture is dedifferentiation of the cells – changing from hyaline to more fibroblastic-like phenotype. After using 2-dimensional collagen/fibrin scaffold seeded with cells, which could not maintain chondrocyte phenotype, we are now examining the potential of alginate/agarose hydrogel. The phenotype status of the cells is monitored by a real-time PCR assay which enables the observation of changes in gene expression patterns. The results of our study suggest that the 3-dimensional environment of the tested scaffold provides suitable environment for chondrocyte redifferentiation.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Risbud MV, Sittinger M (2002) Tissue engineering: advance in vitro cartilage generation. Trends Biotechnol 20: 351–356
Hardingham T, Tew S, Murdoch A (2002) Tissue engineering: chondrocytes and cartilage. Arthritis Res 4 (suppl 3): S63-S68
Brittberg M, Lindahl A, Nilsson A, Ohisson C, Isaksson O, Peterson L (1994) Treatement of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 331: 889–895
Radosavljevic D, Drobnic M, Koritnik B, Gorensek M, Pavlovcic V (2005) Clinical overview of the ACI treated patients in the knee over 10 years. Cartilage weekend. 3rd symposium of recent advances in cartilage repair and tissue engineering. Portoroz, Slovenia
Benya PD, Padilla SR, Nimni ME (1978) Independent regulation of collagen typesby chondrocytes during the loss of differentiated function in culture. Cell 15: 1313–1321
Kuettner KE, Memoli VA, Pauli BU, Wrobel NC, Thonar EJ, Daniel JC (1982) Synthesis of cartilage matrix by mammalian chondrocytes in vitro. II. Maintenance of collagen and proteoglycan phenotype. J Cell Biol 93: 751–757
Bonaventure J, Kadhom N, Cohen-Solal L, Ng KH, Bourguignon J, Lasselin C, Freisinger P (1994) Reexpression of cartilage-specific genes by dedifferentiated human articular chondrocytes cultured in alginate beads. Exp Cell Res 212: 97–104
Glowacki J, Trepman E, Folkman J (1983) Cell shape and phenotypic expression in chondrocytes. Proc Soc Exp Biol Med 172: 93–98
Lee DA, Reisler T, Bader DL (2003) Expansion of chondrocytes for tissue engineering in alginate beads enhances chondrocytic phenotype compared to conventional monolayer techniques. Acta Orthop Scand 74: 6–15
Chubinskaya S, Huch K, Schulze M, Otten L, Aydelotte MB, Cole AA (2001) Gene expression by human articular chondrocytes cultured in alginate beads. J Histochem Cytochem 49: 1211–1219
Benya PD, Shaffer JD (1982) Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 1: 1313–1321
Marlovits S, Hombauer M, Tamandl D, Vecsei V, Schlegel W (2004) Quantitative analysis of gene expression in human articular chondrocytes in monolayer culture. Int J Mol Med 13: 281–287
Darling EM, Athanasiou KA (2005) Rapid phenotypic changes in passaged articular chondrocyte subpopulations. J Orthoped Res 23: 425–432
Martin I, Jakob M, Schafer D, Dick W, Spagnoli G, Heberer M (2001) Quantitative analysis of gene expression in human articular cartilage from normal and osteoarthritic joints. Osteoarthritis Cartilage 9: 112–118
Drobnic M, Mars T, Alibegovic A, Bole V, Balazic J, Grubic Z, Brecelj J (2005) Viability of human chondrocytes in an ex vivo model in relation to temperature and cartilage depth. Folia Biol (Praha) 51: 103–108
Dell'Accio F, De Bari C, Luyten FP (2001) Molecular markers predictive of the capacity of expanded human articular chondrocytes to form stable cartilage in vivo. Arthritis Rheum 44: 1608–1619 A. Barlic, D. Radosavljevic, M. Drobnic and N. Kregar-Velikonja
Peterson L, Minas T, Brittberg M, Nilsson A, Sjogren-Jansson E, Lindahl A (2000) Two- to 9-year outcome after autologous chondrocyte transplantation of the knee. Clin Orthop 374: 212–234
Gaissmaier C, Fritz J, Krackhardt T, Flesch I, Aicher WK, Ashammakhi N (2005) Effect of human platelet supernatant on proliferation and matrix synthesis of human articular chondrocytes in monolayer and three-dimensional alginate cultures. Biomaterials 26: 1953–1960
Grunder T, Gaissmaier C, Fritz J, Stoop R, Hortschansky P, Mollenhauer J, Aicher WK (2004) Bone morphogenetic protein (BMP)-2 enhances the expression of type II collagen and aggrecan in chondrocytes embedded in alginate beads. Osteoarthritis Cartilage 12: 559–567
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Barlic, A., Radosavljevic, D., Drobnic, M., Kregar-Velikonja, N. (2007). Advancing in the quality of the cells assigned for Autologous Chondrocyte Implantation (ACI) method. In: Jarm, T., Kramar, P., Zupanic, A. (eds) 11th Mediterranean Conference on Medical and Biomedical Engineering and Computing 2007. IFMBE Proceedings, vol 16. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-73044-6_62
Download citation
DOI: https://doi.org/10.1007/978-3-540-73044-6_62
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-73043-9
Online ISBN: 978-3-540-73044-6
eBook Packages: EngineeringEngineering (R0)