Chapter

Cell and Tissue Reaction Engineering

Part of the series Principles and Practice pp 173-259

Bioreactor Design and Scale-Up

  • G. CatapanoAffiliated withDepartment of Chemical Engineering and Materials, University of Calabria
  • , P. CzermakAffiliated withInstitute of Biopharmaceutical Technology, University of Applied Sciences Giessen-FriedbergDepartment of Chemical Engineering, Kansas State University
  • , R. EiblAffiliated withDepartment of Life Sciences and Facility Management, Institute of Biotechnology, Zurich University of Applied Sciences
  • , D. EiblAffiliated withDepartment of Life Sciences and Facility Management, Institute of Biotechnology, Zurich University of Applied Sciences
  • , R. PörtnerAffiliated withDepartment for Life Sciences and Facility Management, Institute for Biotechnology Zurich University of Applied SciencesInstitute of Bioprocess and Biosystems Engineering, Hamburg University of Technology (TUHH)

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Abstract

Design and selection of cell culture bioreactors are affected by cell-specific demands, engineering aspects, as well as economic and regulatory considerations. Mainly, special demands such as gentle agitation and aeration without cell damage, a well controlled environment, low levels of toxic metabolites, high cell and product concentrations, optimized medium utilization, surface for adherent cells, and scalability have to be considered. This chapter comprises engineering aspects of bioreactor systems (design, operation, scale-up) developed or adapted for cultivation of mammalian cells, such as bioreactors for suspension culture (stirred-tank reactors, bubble columns, and air-lift reactors), fixed bed and fluidized bed reactors, hollow fiber and membrane reactors, and, finally, disposable bioreactors. Aspects relevant for selection of bioreactors are discussed. Finally, an example is given of how to grow mammalian suspension cells from cryopreserved vials to laboratory and pilot scale.