Brain contusions induce a strong local overexpression of MMP-9. Results of a pilot study

  • A. Vilalta
  • J. Sahuquillo
  • M. A. Poca
  • J. De Los Rios
  • E. Cuadrado
  • A. Ortega-Aznar
  • M. Riveiro
  • J. Montaner
Conference paper

DOI: 10.1007/978-3-211-85578-2_81

Part of the Acta Neurochirurgica Supplementum book series (NEUROCHIRURGICA, volume 102)
Cite this paper as:
Vilalta A. et al. (2008) Brain contusions induce a strong local overexpression of MMP-9. Results of a pilot study. In: Steiger H.J. (eds) Acta Neurochirurgica Supplements. Acta Neurochirurgica Supplementum, vol 102. Springer, Vienna

Background Brain contusions are inflammatory evolutive lesions that induce intracranial pressure increase and edema, contributing to neurological outcome. Matrix metal-loproteinases (MMPs) 2 and 9 can degrade the majority of the extracellular matrix components, and are implicated in blood-brain barrier disruption and edema formation. The aim of this study was to investigate MMP-2 and MMP-9 profiles in human brain contusions using zymography.

Methods A prospective study was conducted in 20 traumatic brain injury patients where contusion brain tissue was resected. Brain tissues from lobectomies were used as controls. Brain homogenates were analysed by gelatin zymography and in situ zimography was performed to confirm results, on one control and one brain contusion tissue sample.

Findings MMP-2 and MMP-9 levels were higher in brain contusions when compared to controls. MMP-9 was high during the first 24 hours and at 48 to 96 hours, whereas MMP-2 was slightly high at 24 to 96 hours. In situ zymography confirmed gelatin zymography results. A relation between outcome and MMP-9 levels was found; MMP-9 levels were higher in patients with worst outcome. Conclusions Our results indicate strong time-dependent gelatinase expression primarily from MMP-9, suggesting that the inflammatory response induced by focal lesions should be considered as a new therapeutic target.

Keywords

Neuroinflammation Matrix metalloproteinases Traumatic brain injury Brain contusion Zymography 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag/Wien 2008

Authors and Affiliations

  • A. Vilalta
    • 1
  • J. Sahuquillo
    • 2
  • M. A. Poca
    • 2
  • J. De Los Rios
    • 1
  • E. Cuadrado
    • 3
  • A. Ortega-Aznar
    • 4
  • M. Riveiro
    • 5
  • J. Montaner
    • 3
  1. 1.Neurosurgery and Neurotraumatology Research Unit (UNINN)Vall d'Hebron University Hospital, Universitat Autònoma de BarcelonaBarcelonaSpain
  2. 2.Department of Neurosurgery, Vall d'Hebron University HospitalUniversitat Autònoma de BarcelonaBarcelonaSpain
  3. 3.Neurovascular Research Laboratory and Neurovascular Unit, Vall d'Hebron University HospitalUniversitat Autònoma de BarcelonaBarcelonaSpain
  4. 4.Department of Pathology (Neuropathology), Vall d'Hebron University HospitalUniversitat Autònoma de BarcelonaBarcelonaSpain
  5. 5.Neurotraumatology Intensive Care Unit, Vall d'Hebron University HospitalUniversitat Autònoma de BarcelonaBarcelonaSpain

Personalised recommendations