Chapter

Effectors of Thermogenesis

Volume 32 of the series Experientia Supplementum pp 89-93

The Identification of the Component in the Inner Membrane of Brown Adipose Tissue Mitochondria Responsible for Regulating Energy Dissipation

  • David G. NichollsAffiliated withDepartments of Psychiatry and Biochemical Medicine, Ninewells Medical School, Dundee University
  • , Vibeke S. M. BernsonAffiliated withWennergrens Institut, Stockholm Universitet
  • , Gillian M. HeatonAffiliated withDepartments of Psychiatry and Biochemical Medicine, Ninewells Medical School, Dundee University

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Abstract

The proton conductance of the inner membrane of hamster brown adipose tissue mitochondria can be regulated in vitro by exogenous purine nucleotides, which “bind to a component on the outer face of the inner membrane. This unique mechanism has been proposed to represent the molecular site of non-shivering thermogenesis in this tissue. Using a photo-affinity analogue of ATP, we have identified the nucleotide binding component as a protein of 32,000 daltons.