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Hydrogen is the element of greatest abundance in the universe; however, its production from renewable resources remains a major challenge. Biohydrogen produced from biorenewables is a promising alternative for a sustainable energy source. Biohydrogen is a renewable biofuel produced from biorenewable feedstocks by chemical, thermochemical, biological, biochemical, and biophotolytical methods. As a sustainable energy supply with minimal or zero use of hydrocarbons, hydrogen is a promising alternative to fossil fuel. It is a clean and environmentally friendly fuel, which produces water instead of greenhouse gases when combusted.