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Tissue Engineering in Regenerative Medicine pp 39–52Cite as

Current Status of Induced Pluripotent Stem Cells

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Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

The discovery of induced pluripotent stem cells (iPSCs) has “spiced up” the stem cell research field in the last few years. It has made tremendous progress in a very short time by demonstrating that adult fibroblasts could be reprogrammed into iPSCs using pluripotency factors. This suggested that cell fates are not as permanent as initially thought, but rather possess a degree of plasticity. Unsurprisingly, induced pluripotent stem cell technology still faces many technical obstacles before safe and high-quality human iPSCs can be generated for therapeutic applications. This chapter examines the current status of iPSC technology and new methods for ­inducing pluripotency and its use in modeling human disease.

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Abbreviations

iPSCs:

Induced pluripotent stem cells

ESCs:

Embryonic stem cells

ICM:

Inner cell mass

SCNT:

Somatic cell nuclear transfer

MEFs:

Mouse embryonic fibroblasts

OSKM:

Oct4, Sox2, Klf4, and c-Myc

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Acknowledgments

We apologize to colleagues whose work could not be cited due to space limitations. T.H. is supported by a stem cell research internship program of the California Institute for Regenerative Medicine and California State University at Long Beach. W.L. is supported by a postdoctoral fellowship from the California Institute for Regenerative Medicine. Y.S. is supported by the National Institutes of Health/NINDS (R01 NS059546 and RC1 NS068370) and the California Institute for Regenerative Medicine (TR2-01832).

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Authors and Affiliations

  1. Department of Neurosciences, Center for Gene Expression and Drug Discovery, Beckman Research Institute of City of Hope, Duarte, CA, USA

    Thach-Vu Ho, Grace Asuelime, Wendong Li & Yanhong Shi

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  1. Thach-Vu Ho
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  2. Grace Asuelime
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  3. Wendong Li
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Correspondence to Yanhong Shi .

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  1. Eli and Edythe Broad Center of Regenerat, Department of Pediatrics, UCSF, Cardiovascular Research Institute, 513 Parnassus Avenue HSE 1422, San Francisco, 94143, USA

    Harold S. Bernstein

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Ho, TV., Asuelime, G., Li, W., Shi, Y. (2011). Current Status of Induced Pluripotent Stem Cells. In: Bernstein, H. (eds) Tissue Engineering in Regenerative Medicine. Stem Cell Biology and Regenerative Medicine. Humana Press. https://doi.org/10.1007/978-1-61779-322-6_3

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