Macrophages and Natural Killer Cells

Volume 155 of the series Advances in Experimental Medicine and Biology pp 261-266

Regulation of Bone-Marrow Macrophage Proliferation

  • David A. HumeAffiliated withSir William Dunn School of Pathology
  • , Siamon GordonAffiliated withSir William Dunn School of Pathology

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Macrophages are derived from pluripotent bone marrow stem cells (1). Their proliferation and differentiation can be induced in vitro by the addition of “conditioned media” that contain colony-stimulating factors (2–6). Colony-stimulating factors (CSF) are normally assayed by their ability to promote colony formation from stem cells in semi-solid (soft agar or methylcellulose) medium. Two types of colony-stimulating factors that influence macrophage growth have been identified. CSF-1 (isolated from mouse L-cell conditioned medium or human urine) gives rise to colonies entirely composed of macrophages while CSF-II (e.g. from WEHI-3 conditioned medium or endotoxinlung conditioned medium) yields mixed colonies of granulocytes and macrophages (2–6).