Antagonism of the Lethal Effects of Cyanide with Resealed Erythrocytes Containing Rhodanese and Thiosulfate

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Abstract

These studies are concerned with the application of resealed erythrocytes containing rhodanese (Cyanide: Thiosulfate Sulfurtransferase, E.C.2.8.1.1.) in cyanide antagonism. In earlier studies, we reported that resealed murine erythrocytes containing rhodanese and sodium thiosulfate were able to convert cyanide to the less toxic thiocyanate in vitro,.1 Subsequently, it was reported that these resealed erythrocytes retained their cyanide detoxifying properties in the intact animal.2 When mice received these resealed erythrocytes containing rhodanese and thiosulfate, they demonstrated decreased blood cyanide concentrations, and increased thiocyanate formation. Moreover, rhodanese activity within these resealed erythrocytes appears to be unaltered by the intracellular RBC environment. Although it is possible to demonstrate the disappearance of blood cyanide, increased formation of thiocyanate, reversal of cytochrome oxidase inhibition, and methemoglobin sequestion of cyanide as cyanmethemoglobin, these biochemical parameters, which have been used in the past as predictive models, do not always correlate well with the antagonism of the lethal effect of cyanide.