Lectin Cross-Linking Interactions with Multivalent Carbohydrates
- C. Fred Brewer
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The present findings provide a molecular basis for a new paradigm of specificity in multivalent carbohydrate-lectin interactions, namely the formation of type 2 homogeneous cross-linked lattices between multivalent carbohydrates and lectins. The present x-ray data demonstrate that the cross-linked complexes formed between a series of structurally related divalent carbohydrates and a single tetravalent lectin (SBA) are distinct and due to crystal packing interactions. These results thus provide a molecular basis for the formation of homogeneous type 2 cross-linked complexes between lectins and multivalent carbohydrates and glycoconjugates.
These findings are also relevant to the observations that lectin-carbohydrate cross-linking interactions are involved in cellular recognition and signal transduction processes. For example, activated human T-cells undergo apoptosis due to binding and cross-linking of specific glycoprotein receptors by galectin-1 (Pace et al., 1999). Confocal microscopy shows that the galectin cross-linked glycoprotein receptors form homogeneous aggregates from a population of previously dispersed molecules on the surface of the cells. The crystal structures of the four SBA/pentasaccharide complexes thus repesent models for lectin-carbohydrate clustering in vivo.
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- Lectin Cross-Linking Interactions with Multivalent Carbohydrates
- Book Title
- The Molecular Immunology of Complex Carbohydrates —2
- pp 17-25
- Print ISBN
- Online ISBN
- Series Title
- Advances in Experimental Medicine and Biology
- Series Volume
- Series ISSN
- Springer US
- Copyright Holder
- Kluwer Academic / Plenum Publishers, NewYork
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- Albert M. Wu (1) (2)
- Editor Affiliations
- 1. Glyco Research Laboratory Institutes of Basic Medical Sciences and Natural Products, College of Medicine Chang-Gung University
- C. Fred Brewer (3)
- Author Affiliations
- 3. Departments of Molecular Pharmacology, Microbiology and Immunology Albert Einstein College of Medicine, New York, Bronx, 10641, USA
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