Chapter

Oxygen Transport To Tissue XXIII

Volume 510 of the series Advances in Experimental Medicine and Biology pp 199-204

Probing Rat Brain Oxygenation with Near-Infrared Spectroscopy (NIRS) and Magnetic Resonance Imaging (MRI)

  • Yu ChenAffiliated withDepartment of Biochemistry and Biophysics, University of PennsylvaniaDepartment of Bioengineering, University of Pennsylvania
  • , Xavier IntesAffiliated withDepartment of Biochemistry and Biophysics, University of Pennsylvania
  • , Dharmesh R. TailorAffiliated withDepartment of Bioengineering, University of PennsylvaniaDepartment of Radiology, University of Pennsylvania
  • , Ravinder R. RegatteAffiliated withDepartment of Radiology, University of Pennsylvania
  • , HongYan MaAffiliated withDepartment of Biochemistry and Biophysics, University of Pennsylvania
  • , Vasilis NtziachristosAffiliated withDepartment of Biochemistry and Biophysics, University of Pennsylvania
  • , John S. LeighAffiliated withDepartment of Radiology, University of Pennsylvania
  • , Ravinder ReddyAffiliated withDepartment of Radiology, University of Pennsylvania
  • , Britton ChanceAffiliated withDepartment of Biochemistry and Biophysics, University of Pennsylvania

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Abstract

Near-infrared spectroscopy (NIRS) has been a promising technique for non-invasively quantifying tissue chromophores concentrations. One of the potential applications is the monitoring of cerebral blood volume and blood oxygenation saturation. Theories and instruments have been developed to improve the measurement sensitivity and accuracy“. In vivo studies have been performed with calibration of NIRS saturation using the weighted average of global arterial and venous saturations. Comparison of NIRS results with other non-invasive imaging modalities such as MRI can provide more information on the co-localized region, and has attracted a lot of interest recently. Several studies have reported the temporal correlation between the fMRI BOLD (blood oxygenation level dependent) signal and deoxy-hemoglobin concentration measured by NIRS. To our knowledge, the quantitative relation has not been reported. In this study, we show the correlation of deoxy-hemoglobin concentration as well as oxygen saturation measured with NIRS and the conventional T2*-weighted BOLD MM signal for an in vivo rat model during inhalation of gas with different concentrations of oxygen (FiO2).