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Oxygen Transport to Tissue XXXV

Volume 789 of the series Advances in Experimental Medicine and Biology pp 243-249

Date:

Mapping the Redox State of CHOP-Treated Non-Hodgkin’s Lymphoma Xenografts in Mice

  • He. N. XuAffiliated withDepartment of Radiology, University of PennsylvaniaBritton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania
  • , Tahreem A. MirAffiliated withDepartment of Radiology, University of PennsylvaniaBritton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania
  • , Seung-Cheol LeeAffiliated withDepartment of Radiology, University of Pennsylvania
  • , Min FengAffiliated withDepartment of Radiology, University of PennsylvaniaBritton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania
  • , Namisa FarhadAffiliated withDepartment of Radiology, University of PennsylvaniaBritton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania
  • , Regine ChoeAffiliated withDepartment of Biomedical Engineering, University of Rochester
  • , Jerry D. GlicksonAffiliated withDepartment of Radiology, University of Pennsylvania
  • , Lin Z. LiAffiliated withDepartment of Radiology, University of PennsylvaniaBritton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, University of PennsylvaniaAbramson Cancer Center, University of PennsylvaniaInstitute of Translational Medicine and Therapeutics, University of Pennsylvania Email author 

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Abstract

Drug treatment may alter the metabolism of cancer cells and may alter the mitochondrial redox state. Using the redox scanner that collects the fluorescence signals from both the oxidized flavoproteins (Fp) and the reduced form of nicotinamide adenine dinucleotide (NADH) in snap-frozen tumor tissues, we investigated the effects of chemotherapy on mouse xenografts of a human diffuse large B-cell lymphoma cell line (DLCL2). The mice in the treatment group were treated with CHOP – cyclophosphamide (C) + hydroxydoxorubicin (H) + Oncovin (O) + prednisone (P) using the following regimen: CHO administration on day 1 followed by prednisone administration on day 1–5. On day 5 the mitochondrial redox state of the treated group was slightly more reduced than that of the control group (p = 0.049), and the Fp content of the treated group was significantly decreased (p = 0.033).