Triplet Imaging of Oxygen Consumption During the Contraction of a Single Smooth Muscle Cell (A7r5)

  • Matthias Geissbuehler
  • Thiemo Spielmann
  • Aurélie Formey
  • Iwan Märki
  • Marcel Leutenegger
  • Boris Hinz
  • Kai Johnsson
  • Dimitri Van De Ville
  • Theo Lasser
Conference paper

DOI: 10.1007/978-1-4614-1566-4_39

Part of the Advances in Experimental Medicine and Biology book series (volume 737)
Cite this paper as:
Geissbuehler M. et al. (2012) Triplet Imaging of Oxygen Consumption During the Contraction of a Single Smooth Muscle Cell (A7r5). In: Wolf M. et al. (eds) Oxygen Transport to Tissue XXXIII. Advances in Experimental Medicine and Biology, vol 737. Springer, New York, NY

Abstract

Triplet imaging is a novel optical technique that allows investigating oxygen metabolism at the single cell and the sub-cellular level. The method combines high temporal and spatial resolutions which are required for the monitoring of fast kinetics of oxygen concentration in living cells. Calibration and validation are demonstrated with a titration experiment using l-ascorbic acid with the enzyme ascorbase oxidase. The method was applied to a biological cell system, employing as reporter a cytosolic fusion protein of β-galactosidase with a SNAP-tag labeled with tetramethylrhodamine. Oxygen consumption in single smooth muscle cells A7r5 during an [Arg8]-vasopressin-induced contraction is measured. The triplet lifetime images over time can be related to an intracellular oxygen consumption corresponding to a mono-exponentially decaying intracellular oxygen concentration. This is in good agreement with previously reported measurements of oxygen consumption in skeletal muscle fibers.

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Matthias Geissbuehler
    • 1
  • Thiemo Spielmann
    • 1
    • 2
  • Aurélie Formey
    • 3
  • Iwan Märki
    • 1
  • Marcel Leutenegger
    • 1
    • 4
  • Boris Hinz
    • 5
  • Kai Johnsson
    • 6
  • Dimitri Van De Ville
    • 7
    • 8
  • Theo Lasser
    • 1
  1. 1.Laboratoire d’Optique Biomédicale LOBÉcole Polytechnique Fédérale de Lausanne (EPFL)LausanneSwitzerland
  2. 2.Experimental Biomolecular Physics, Department of Applied Physics, Royal Institute of TechnologyAlbaNova University CenterStockholmSweden
  3. 3.Laboratory of Cell Biophysics LCBÉcole Polytechnique Fédérale de Lausanne (EPFL)LausanneSwitzerland
  4. 4.Department of NanoBiophotonicsMax Planck Institute for Biophysical ChemistryGöttingenGermany
  5. 5.Laboratory of Tissue Repair and Regeneration, CIHR Group in Matrix DynamicsUniversity of TorontoTorontoCanada
  6. 6.Laboratory of Protein Engineering LIP1École Polytechnique Fédérale de Lausanne (EPFL)LausanneSwitzerland
  7. 7.Medical Image Processing Laboratory MIPÉcole Polytechnique Fédérale de Lausanne (EPFL)LausanneSwitzerland
  8. 8.Medical Image Processing Laboratory MIPUniversity of GenevaGenevaSwitzerland

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