Abstract
The sea urchin, Toxopneustes pileolus (Lamarck), a member of the family Toxopneustidae, is found on the sea bed in the Indo-Pacific area. It possesses extremely well-developed globiferous pedicellariae which contain pharmacologically active substances and cause deleterious effects (Fujiwara, 1935; Okada., 1955a; Kimura et al., 1975; Nakagawa et al., 1982, 1991 and 1992; Takei et al., 1993). It is suggested that a sea urchin having large pedicellariae is more dangerous than one having numerous small pedicellariae (Halstead, 1987). Okada et al. (1955b) reported that there were sub-types of globiferous pedicellariae on the external surface of T. pileolus: ordinary pedicellaria, trumpet pedicellaria and giant pedicellaria. We have also observed the trumpet pedicellaria (globiferous pedicellaria) and giant pedicellaria (large globiferous pedicellaria). In the course of comparative study on venom proteins from the globiferous pedicellariae and large globiferous pedicellariae of T. pileolus, we found that the crude venom extracts from the pedicellariae of the two sub-types caused agglutination with mouse erythrocytes in a Ca2+-independent manner
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References
Bradford, M. M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem.72: 248–254.
Drickamer, K., 1988, Two distinct classes of carbohydrate-recognition domains in animal lectins. J. Biol. Chem.263: 9557–9560.
Fujiwara, T., 1935, On the poisonous pedicellaria of Toxopneustes pileolus(Lamarck). Annot. Zool. Jpn.15: 62–69.
Giga, Y., Sutoh, K., and Ikai, A., 1985, A new multimeric hemagglutinin from the coelomic fluid of the sea urchin Anthocidaris crassispina. Biochemistry. 24: 4461–4467.
Giga, Y., Ikai, A., and Takahashi, K., 1987, The complete amino acid sequence of echinoidin, a lectin from the coelomic fluid of the sea urchin Anthocidais crassispina. J. Biol. Chem. 262: 6197–6203.
Halstead, B. W., 1988, Poisonous and Venomous Marine Animals of the World, pp. 196-212. Darwin Press, Princeton.
Hatakeyama, T., Himeshima,T., Komatsu, A., and Yamasaki, N., 1993, Purification and characterization of two lectins from the sea cucumber Strichopus japonicus. Biosci. Biotech. Biochem.57: 1736–1739.
Hatakeyama, T., Kohzaki, H., Nagatomo, H., and Yamasaki, N., 1994, Purification and characterization of four Ca2+-dependent lectins from the marine invertebrate, Cucumaria echinata. J. Biochem.116: 209–214.
Kawagishi, H., Yamawaki, M., Isobe, S., Usui, T., Kimura, A., and Chiba, S., 1994, Two lectins from the marine sponge Halichondria akada: An N-acetyl-sugar-specific lectin (HOL-I) and an N-acetyllactosamine-specific lectin (HOL-II). J. Biol. Chem.269: 1375–1379.
Kimura, A., Hayashi, H., and Kuramoto, M., 1975, Studies of urci-toxins: Separation, purification and pharmacological actions of toxinic substances. Japan J. Pharmacol.25: 109–120.
Laemmli, U, K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680–685.
Muramoto, K., and Kamiya, H., 1986, The amino-acid sequence of a lectin of the acorn barnacle Megabalanus rosa. Biochim. Biophys. Acta. 874: 285–295.
Nakagawa, H., Kimura, A., Takei, M., and Endo, K., 1982, Histamine release from rat mast cells induced by an extract from the sea urchin Toxopneustes pileolus. Toxicon. 20: 1095–1097.
Nakagawa, H., Tu, A. T., and Kimura, A., 1991, Purification and characterization of Contractin A from the pedicellarial venom of sea urchin, Toxopneustes pileolus. Arch. Biochem. Biophys.284: 279–284.
Nakagawa, H., Yanagihara, N., Izumi, F., Wada, A., and Kimura, A., 1992, Inhibition of nicotinic acetylcholine receptor-mediated secretion and synthesis of catecholamines by sea urchin toxin in cultured bovine adrenal medullary cells. Biochem. Pharmacol.44: 1779–1785.
Nakagawa, H., Tomihara, Y., Araki, Y., and Hayashi, H., 1994, Preliminary studies on venom proteins in the pedicellariae of the toxopneustid sea urchins, Toxopneustes pileolus and Tripneustes gratilla. J. Natural Toxins3: 25–34.
Ohura, K., Shinohara, M., Ogata, K., Nishiyama, A., and Mori, M., 1990, Leucocyte function in rats with naturally occurring gingivitis. Archs oral Biol. 35: Suppl. 185s–187s.
Okada, K., 1955a, Biological studies on the practical utilities of poisonous marine invertebrates. I. A preliminary note on the toxical substance detected in the trumpet sea urchin, Toxopneustes pileolus. Ree. Oceanogr. Wks.2: 49–52.
Okada, K., Hashimoto, T., and Miyauchi, Y., 1955b, A preliminary report on the poisonous effect of Toxopneustestoxin upon the heart of oyster. ull. Marine Biol. Asamushi. 7: 133–140.
Suzuki, T., Takagi, T., Furukohri, T., Kawamura, K., and Nakauchi, M., 1990, A calcium-dependent galactose-binding lectin from the Tunicate Polyandrocarpa misakiensis: Isolation, characterization, and amino acid sequence. J. Biol, Chem.265: 1274–1281.
Takei, M., Nakagawa, H., and Endo, K., 1993, Mast cell activation by pedicellarial toxin of sea urchin, Toxopneustes pileolus. FEBS Lett.328: 59–62.
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© 1996 Plenum Press, New York
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Nakagawa, H. et al. (1996). Isolation of a Novel Lectin from the Globiferous Pedicellariae of the Sea Urchin Toxopneustes Pileolus . In: Singh, B.R., Tu, A.T. (eds) Natural Toxins 2. Advances in Experimental Medicine and Biology, vol 391. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0361-9_14
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DOI: https://doi.org/10.1007/978-1-4613-0361-9_14
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