Oxygen Transport to Tissue XXXI

Volume 662 of the series Advances in Experimental Medicine and Biology pp 323-328


NIRS Measurement of O2 Dynamics in Contracting Blood and Buffer Perfused Hindlimb Muscle

  • Kazumi MasudaAffiliated withFaculty of Human Sciences, Kanazawa University Email author 
  • , Hisashi TakakuraAffiliated withThe Graduate School of Natural Science and Technology, Kanazawa University
  • , Yasuro FuruichiAffiliated withThe graduate School of Education, Kanazawa University
  • , Satoshi IwaseAffiliated withDepartment of Physiology, Aichi Medical University
  • , Thomas JueAffiliated withDepartment of Biochemistry and Molecular Medicine, University of California Davis

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In order to obtain evidence that Mb releases O2 during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (Δ[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of Δ[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the Δ[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO2 as workload increases.