Structure, Processing, and Polymerization of Rainbow Trout Egg Vitelline Envelope Proteins
Mammalian and non-mammalian eggs are surrounded by a zona pellucida (ZP) and vitelline envelope (VE), respectively. The rainbow trout egg VE consists of three proteins, VEα, VEβ, and VEγ, which are related to mouse egg ZP proteins ZP1, ZP2, and ZP3. Mass spectrometry (MS) has been used extensively to identify the intramolecular disulfide linkages and the cellular site of proteolytic processing of trout VE proteins. Additionally, Blue Native-PAGE (BN-PAGE) has been used to investigate polymerization of purified trout VE proteins under non-denaturing conditions. Results of these experiments reveal that, despite ~400 million years separating the appearance of trout and mice, and the change from external to internal fertilization and development, VE and ZP proteins have a great deal in common.
- Structure, Processing, and Polymerization of Rainbow Trout Egg Vitelline Envelope Proteins
- Book Title
- Applications of Mass Spectrometry in Life Safety
- Book Part
- pp 23-36
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- Online ISBN
- Series Title
- NATO Science for Peace and Security Series A: Chemistry and Biology
- Series ISSN
- Springer Netherlands
- Copyright Holder
- Springer Science+Business Media B.V.
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- Editor Affiliations
- 1. DWI e.V. and Institute of Technical and Macromolecular Chemistry, RWTH Aachen University
- 2. University “Aurel Vlaicu”
- Author Affiliations
- 3. Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY, 10029-6574
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