Genetic Engineering of Strawberry for Taste Improvement and Enhanced Disease Resistance by Introduction of thauII Gene
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- Schestibratov K.A., Dolgov S.V. (2007) Genetic Engineering of Strawberry for Taste Improvement and Enhanced Disease Resistance by Introduction of thauII Gene. In: Xu Z., Li J., Xue Y., Yang W. (eds) Biotechnology and Sustainable Agriculture 2006 and Beyond. Springer, Dordrecht
Strawberry is an important small fruit crop throughout the world, and its production is growing steadily. Gray mold or Botrytis fruit rot, caused by Botrytis cinerea, is one of the destructive diseases of strawberry responsible for substantial yield loss before and after harvest. The taste attributes of strawberries (Fragaria x ananassa Duch.) are regarded as another significant quality factors by both consumers and the food industry. Nineteen independent transgenic lines of cv. Firework and 15 lines of cv. Selekta were obtained via Agrobacterium-mediated transformation. Accumulation of thaumatin II was detected by Western blotting analysis. Most of tested F-40-I and S-40-I transgenic lines expressing thaumatin II protein showed significantly smaller area of the necrotic lesions observed on the leaf disks. Organoleptic analysis of transgenic fruits demonstrated sweetness improvement in 8 F-40-I and 5 S-40-I lines accumulating thaumatin II protein.
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