Mass propagation of elite trees for commercial use as well as for the extraction of chemically consistent medicinal preparations requires that the plants be grown in an environment with consistent light, nutrition and temperature as well as in isolation from abiotic and biotic contaminants (Murch et al., 2001; Saxena, 2001).This chapter provides details of various steps involved in the micropropagation of C. cujete including: 1. establishment of in vitro seedling cultures of C. cujete, 2. development of protocols for proliferation and maintenance of axenic cultures in perpetuity, 3. protocols for de novo regeneration, and 4. determination of the optimal configuration of bioreactors for large-scale production.
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Liu, C.Z., He, S.S., Romero, R., Murch, S.J., Saxena, P.K. (2007). Micropropagation of Calabash tree Crescentia Cujete L. In: Jain, S.M., Häggman, H. (eds) Protocols for Micropropagation of Woody Trees and Fruits. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6352-7_39
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