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Genetics and Genomics of the Triticeae

Volume 7 of the series Plant Genetics and Genomics: Crops and Models pp 163-199

Date:

Methods for Genetic Analysis in the Triticeae

  • Abraham KorolAffiliated withInstitute of Evolution, Faculty of Science, University of Haifa Email author 
  • , David MesterAffiliated withDepartment of Crop Genetics, John Innes Centre, Norwich Research Park
  • , Zeev FrenkelAffiliated withInstitute of Evolution, Faculty of Science, University of Haifa
  • , Yefim RoninAffiliated withInstitute of Evolution, Faculty of Science, University of Haifa

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Abstract

The objective of genetic analysis is to reveal genome structural and functional organization. One of the major tools developed at early stages of genetics was genetic mapping. Genetic maps are a very important tool in evolutionary genomics and numerous practical applications like breeding, medical genetics, and gene cloning. An important usage of multilocus maps is genetic dissection of quantitative traits, or mapping quantitative trait loci (QTL). Fine QTL mapping is a prerequisite for efficient marker-assisted selection and map-based cloning. However, the fine mapping challenge, especially if the target is a gene of weak or moderate effect, requires large sample sizes and dense maps. New array-based technologies (SNP and tilling arrays) partially solve this problem but at a very high project-wise genotyping cost. This is why despite some technical obstacles, genetic analysis based on selective genotyping and selective DNA pooling becomes very popular, especially in human genetics. In this chapter we consider methods for building genetic maps (Section 6.1), various versions of “multiple” approach for QTL mapping (Section 6.2), and a new cost-effective method for genetic mapping based on selective DNA pooling (Section 6.3). Whenever possible, the examples are based on Triticeae species.