Handbook Of Biological Confocal Microscopy

pp 207-220

The Intermediate Optical System of Laser-Scanning Confocal Microscopes

  • Ernst H. K. StelzerAffiliated withLight Microscopy Group, European Molecular Biology Laboratory (EMBL)

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This text explains some of the basics of intermediate optical systems in confocal microscopes. Important microscope components are discussed in more detail in other chapters: lasers (Chapter 5) and other light sources (Chapter 6), objective lenses (Chapter 7), special optical elements (Chapter 3), fibers (Chapter 26), and detectors (Chapter 12). Although this chapter concentrates on laser-based single-spot scanning instruments while other types of instruments are covered elsewhere (Chapters 10 and 29), remarks on different approaches will guide the interested reader.