Handbook Of Biological Confocal Microscopy

pp 162-206

The Contrast Formation in Optical Microscopy

  • Ping-Chin ChengAffiliated withState University of New York at BuffaloNational University of Singapore

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In any form of microscopy, one needs not only an imaging system with enough resolution to delineate the fine details of the specimen but also a suitable contrast mechanism by which to “see” the shape of the structures of interest. Contrast is the difference between the signal in one pixel and that in another that conveys to the viewer information about the shape of the specimen. It is the difference between a blank screen and an image.