Practical Fluorescence Resonance Energy Transfer or Molecular Nanobioscopy of Living Cells

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Abstract

After formulating this philosophic question in a poetic form, Leonardo the Scientist, provides us with a real experimental (optical) setup. “As I propose to treat the nature of the moon, it is necessary that I first describe the perspective of mirrors, whether plane, concave, or convex,” (B.M.94r – Arundel MS in British Museum). Next, in the pages of Codex Atlanticus (C.A.190r), Leonardo invites us to “Construct the glasses to see the moon magnified” and half a millennium later we are still following him for, as Bulgakov famously said, “Manuscripts do not burn!”